Product Name	Vimentin
Chinese Name	波形蛋白抗体
Alias	FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.
literatures	Specific References  (12)     |     SL23063R has been referenced in 12 publications. [IF=7.419] Honghua Yue. et al. Colchicine: Emerging therapeutic effects on atrial fibrillation by alleviating myocardial fibrosis in a rat model. BIOMED PHARMACOTHER. 2022 Oct;154:113573  IF ;  Rat.   PubMed:35987161 [IF=5.959] Hou L et al. Nontoxic concentration of ochratoxin A decreases the dosage of cyclosporine A to induce chronic nephropathy model via autophagy mediated by toll-like receptor 4. Cell Death Dis. 2020 Feb 27;11(2):153.  IF ;  human.   PubMed:32108135 [IF=5.895] Heng Du. et al. Nontoxic Concentration of Ochratoxin A Aggravates Renal Fibrosis Induced by Adriamycin/Cyclosporine A Nephropathy via TGF-β1/SMAD2/3. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXX  WB ;  Human.   PubMed:36278938 [IF=5.314] Jiajia Xu. et al. Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease. INT J MOL MED. 2022 Dec;50(6):1-13  IF ;  Rat.   PubMed:36300203 [IF=4.292] Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-β/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041  IF ;  Dog.   PubMed:34864206 [IF=4.175] Huajun Wang. et al. LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-β2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis. Cancer Cell Int. 2020 Dec;20(1):1-12  WB ;  Human.   PubMed:33292220 [IF=3.642] Xinru Mao. et al. Selenomethionine alleviated Ochratoxin A induced pyroptosis and renal fibrotic factors expressions in MDCK cells. 2021 Oct 21  WB ;  Dog.   PubMed:34676619 [IF=3.383] Qi H et al. MicroRNA-183 exerts a protective role in lupus nephritis through blunting the activation of TGF-β/Smad/TLR3 pathway via reducing Tgfbr1. Exp Cell Res . 2020 Sep 15;394(2):112138.  WB ;  Mouse&Human.   PubMed:32535034 [IF=3.383] Huimeng Qi. et al. MicroRNA-183 exerts a protective role in lupus nephritis through blunting the activation of TGF-β/Smad/TLR3 pathway via reducing Tgfbr1. Exp Cell Res. 2020 Sep;394:112138  WB ;  Human.   PubMed:32535034 [IF=2.923] Junyuan Ma. et al. Effects of melatonin on development and hormone secretion of sheep theca cells in vitro. THERIOGENOLOGY. 2023 Mar;198:172  IF ;  Sheep.   PubMed:36592515 [IF=2.586] Xiaoyan Song. et al. SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway. TISSUE CELL. 2022 Oct;:101954  WB ;  Human.   PubMed:36270072 [IF=2.586] Yunan Wang. et al. A novel type of mesenchymal stem cells derived from bovine metanephric mesenchyme. TISSUE CELL. 2022 Dec;79:101970  IF ;  Bovine.   PubMed:36370625
Research Area	Tumour  Cell biology  immunology  Developmental biology  Neurobiology  Signal transduction  Stem cells  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	53kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Vimentin: 101-200/466
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009] Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Subunit: Homopolymer assembled from elementary dimers. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain). Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B. Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Post-translational modifications: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Similarity: Belongs to the intermediate filament family. SWISS: P08670 Gene ID: 7431 Database links: Entrez Gene: 7431 Human Entrez Gene: 22352 Mouse Entrez Gene: 81818 Rat Omim: 193060 Human SwissProt: P08670 Human SwissProt: P20152 Mouse SwissProt: P31000 Rat Unigene: 455493 Human Vimentin—波形蛋白。是五种主要的中间丝之一，存在于各种正常和病理性间质来源的组织，如纤维母细胞、endothelial cells、lymphocyte等正常细胞和肉瘤、淋巴瘤、黑色素瘤等Tumour。波形蛋白是负责维持Cytoskeleton完整性的蛋白之一。
Product Picture	Sample: U2os(human)cell Lysate at 40 ug U87mg(human)cell Lysate at 40 ug Hela (human)cell Lysate at 40 ug Jurkat(human)cell Lysate at 40 ug Primary: Anti- Vimentin (SL23063R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53kD Observed band size: 53 kD Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Vimentin (SL23063R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (SL23063R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (SL23063R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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