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Product Name CCR-2 Chinese Name 细胞表面Chemokine受体2抗体 Alias C-C Chemokine receptor 2B; C C chemokine receptor type 2; C C CKR 2; CC chemokine receptor type 2; CC CKR 2; CCCKR2; CCR 2; CCR1L; CCR2A; CCR2B; CCR5L; CD192; CD192 antigen; Chemokine C C motif receptor 2; Chemokine CC Motif Receptor 2; CKR 2; CKR2; CKR2A; CKR2B; CMKBR2; MCP 1 R; MCP1 RECEPTOR; MCP1R; Monocyte chemoattractant protein 1 receptor; Monocyte Chemotactic Protein 1 Receptor; CCR2_MOUSE; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR2; Monocyte chemoattractant protein 1 receptor; MCP-1-R. Research Area immunology Cell Surface Molecule Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 41kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse CCR-2: 301-373/373 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene. [provided by RefSeq, Mar 2009].
Function:
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
Subunit:
Binds to HIV-1 Tat. Interacts with ARRB1.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Post-translational modifications:
N-glycosylated.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P51683
Gene ID:
12772
Database links:Entrez Gene: 729230 Human
Entrez Gene: 12772 Mouse
Omim: 601267 Human
SwissProt: P41597 Human
SwissProt: P51683 Mouse
Unigene: 511794 Human
Unigene: 705362 Human
Unigene: 6272 Mouse
Unigene: 211983 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCR-2) Polyclonal Antibody, Unconjugated (SL23026R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (SL23026R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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