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Product Name PEG3 Chinese Name PEG3蛋白抗体 Alias Paternally-expressed gene 3 protein; Zinc finger and SCAN domain-containing protein 24; Kruppel-type zinc finger protein; paternally expressed 3; Paternally expressed gene 3; PEG3 protein; Pw1; Zn-finger protein Pw1; PEG3_HUMAN; Paternally-expressed gene 3 protein. Research Area Tumour Cell biology Neurobiology Signal transduction Cyclin transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 175kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PEG3: 1011-1110/1588 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.
Function:
Induces apoptosis in cooperation with SIAH1A. Acts as a mediator between p53/TP53 and BAX in a neuronal death pathway that is activated by DNA damage. Acts synergistically with TRAF2 and inhibits TNF induced apoptosis through activation of NF-kappa-B. Possesses a tumor suppressing activity in glioma cells.
Subunit:
Homodimer. Interacts with SIAH1A and SIAH2. Interacts with TRAF2.
Subcellular Location:
Nucleus. Cytoplasm.
Tissue Specificity:
Brain, glial cells, astrocytes, embryo, placenta, testis, ovary and uterus. In the placenta it is found in the layer of villous cytotrophoblast cells while in the ovary it is found in the cells of the ovarian stroma including the thecal layers around the follicles. Expression is highly repressed in glioma cell lines.
Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 12 C2H2-type zinc fingers.
Contains 1 SCAN box domain.
SWISS:
Q9GZU2
Gene ID:
18616
Database links:Entrez Gene: 18616 Mouse
Entrez Gene: 66549 Mouse
SwissProt: O54978 Mouse
SwissProt: Q3URU2 Mouse
SwissProt: Q61138 Mouse
SwissProt: Q6GQS0 Mouse
SwissProt: Q8R5N0 Mouse
SwissProt: Q9QX53 Mouse
Unigene: 7952 Mouse
PEG3蛋白是一种转录因子。在胰腺、脑组织、胎盘、卵巢、睾丸等组织中表达,PEG3蛋白位于The nucleus内。PEG3也是一个凋亡促进因子并可能参与调节Tumour坏死因子(TNF)信号传导通路。Product Picture Paraformaldehyde-fixed, paraffin embedded (Human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PEG3) Polyclonal Antibody, Unconjugated (SL22782R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PEG3) Polyclonal Antibody, Unconjugated (SL22782R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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