Product Name	BHMT
Chinese Name	BHMT抗体
Alias	Betaine homocysteine methyltransferase ; Betaine homocysteine S methyltransferase 1 ; Betaine homocysteine S methyltransferase ; Betaine--homocysteine S-methyltransferase 1 ; BHMT1 ; BHMT1_HUMAN ; EC 2.1.1.5 ; Epididymis secretory sperm binding protein Li 61p ; HEL S 61p.
Research Area	Tumour  Signal transduction  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Sheep, )
Applications	WB=1:500-2000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human BHMT: 310-406/406
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008] Function: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. Subcellular Location: Cytoplasm. Tissue Specificity: Found exclusively in liver and kidney. Similarity: Contains 1 Hcy-binding domain. SWISS: Q93088 Gene ID: 635 Database links: Entrez Gene: 497025 Cow Entrez Gene: 635 Human Entrez Gene: 12116 Mouse Entrez Gene: 81508 Rat Omim: 602888 Human SwissProt: Q5I597 Cow SwissProt: Q93088 Human SwissProt: O35490 Mouse SwissProt: O09171 Rat Unigene: 80756 Human Unigene: 329582 Mouse Unigene: 423099 Mouse Unigene: 11406 Rat
Product Picture	Sample: Lane 1: Mouse Liver Lysates Lane 2: Rat Liver Lysates Primary: Anti-BHMT (SL22698R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kDa Observed band size: 45kDa Paraformaldehyde-fixed, paraffin embedded (Human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BHMT) Polyclonal Antibody, Unconjugated (SL22698R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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