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Product Name ACA11 Chinese Name 拟南芥ACA11抗体 Alias calcium-transporting ATPase; Putative calcium-transporting ATPase 11, plasma membrane-type; Ca(2+)-ATPase isoform 11; autoinhibited Ca2+-ATPase 11(Arabidopsis thaliana); ACA11_ARATH. Product Type Botany抗体 Research Area Botany Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Arabidopsis Thaliana, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 112 kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Arabidopsis thaliana ACA11: 201-300/1025 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail In plant cells, the vacuole functions as a major calcium store. Although a calmodulin-regulated Ca2+-ATPase (ACA4) is known to be present in prevacuolar compartments, the presence of an ACA-type Ca2+-ATPase in the mature vacuole of a plant cell has not been verified. Here we provide evidence that ACA11 localizes to the vacuole membrane. ACA11 tagged with GFP was expressed in stable transgenic plants, and visualized in root cells and protoplasts by confocal microscopy. A Ca2+-ATPase function for ACA11 was confirmed by complementation of yeast mutants.
Function:
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles (By similarity).
Subcellular Location:
Membrane; Multi-pass membrane protein.
Similarity:
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.
SWISS:
Q9M2L4
Gene ID:
824900
Database links:Entrez Gene: 824900 ARATH
SwissProt: Q9M2L4 ARATH
拟南芥
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