Product Name	MYL3
Chinese Name	肌球蛋白轻链3抗体
Alias	Cardiac myosin light chain 1; Slow skeletal Myosin; CMH8; CMLC1; MLC1SB; MLC1V; MYL3; MYL3_HUMAN; Myosin light chain 1; Myosin light chain 1 slow twitch muscle B ventricular isoform; myosin light chain 3 alkali ventricular skeletal slow; Myosin light chain 3; slow-twitch muscle B/ventricular isoform; Ventricular slow twitch myosin alkali light chain; Ventricular/slow twitch myosin alkali light chain; VLC1.
Research Area	Cardiovascular  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:1000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	22kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MLC/Myosin light chain 3: 101-195/195
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker. Function: Regulatory light chain of myosin. Does not bind calcium. Subunit: Myosin is a hexamer of 2 heavy chains and 4 light chains. Post-translational modifications: The N-terminus is blocked. N-terminus is methylated by METTL11A/NTM1. DISEASE: Cardiomyopathy, familial hypertrophic 8 (CMH8)[MIM:608751]: A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Rarely, patients present a variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Contains 3 EF-hand domains. SWISS: P08590 Gene ID: 4634 Database links: Entrez Gene: 4634 Human Entrez Gene: 17897 Mouse Entrez Gene: 24585 Rat Omim: 160790 Human SwissProt: P08590 Human SwissProt: P09542 Mouse SwissProt: P16409 Rat Unigene: 517939 Human Unigene: 7353 Mouse Unigene: 1955 Rat
Product Picture	Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-MYL3 (SL2240R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD Sample: Placenta (Mouse) Lysate at 30 ug Primary: Anti- MYL3(SL2240R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 22kD Observed band size: 22 kD Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(SL2240R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(SL2240R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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