Product Name	VEGFR3
Chinese Name	vascular endothelial cell生长因子受体3抗体
Alias	Vascular endothelial growth factor receptor 3; VEGF Receptor 3; Tyrosine-protein kinase; Flt4; VEGFR3; AI323512; Chy; FLT4; FLT41; LMPH1A; LOC285682; PCL; VEGFR-3; fms-related tyrosine kinase 4; VGFR3_MOUSE.
literatures	Specific References  (5)     |     SL2202R has been referenced in 5 publications. [IF=7.84] Zhuo, Wei, et al. "The CXCL12?CCXCR4 Chemokine Pathway: A Novel Axis Regulates Lymphangiogenesis."Clinical Cancer Research 18.19 (2012): 5387-5398.l  Human, Mouse.   PubMed:22932666 [IF=5.99] Wang, Zhixiong, et al. "CXCL1 from tumor-associated lymphatic endothelial cells drives gastric cancer cell into lymphatic system via activating integrin β1/FAK/AKT signaling." Cancer Letters 385 (2017): 28-38.  IF(ICC) ;  Human.   PubMed:27832972 [IF=2.139] M Csöbönyeiová. et al. Immunohistochemical and Scanning Electron Microscopic Confirmation of the Lymphatic Lacunae in the Uterine Tube Mucosal Folds. What Are the Clinical Implications?. PHYSIOL RES. 2022 Dec 27;71(Suppl 1):S115-S123  IHC ;  Human.   PubMed:36592447 [IF=2.136] Hayashi KG et al. Temporal expression and localization of vascular endothelial growth factor family members in the bovine uterus during peri-implantation period. Theriogenology. 2019 Apr 24;133:56-64.  IHC-P ;  Cow.   PubMed:31059929 [IF=2.02] Wang, Zheng, et al. "RhGH attenuates ischemia injury of intrahepatic bile ducts relating to liver transplantation." Journal of Surgical Research 171.1 (2011): 300-310.  IHC-P ;  Rat.   PubMed:20462597
Research Area	Tumour  immunology  Signal transduction  Growth factors and hormones  Kinases and Phosphatases  The cell membrane受体  vascular endothelial cell
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	151kDa
Cellular localization	The nucleus cytoplasmic The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human VEGFR-3: 901-1000/1298 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells Function: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'. Subunit: Interacts with VEGFC and VEGFD. Monomer in the absence of bound VEGFC or VEGFD. Homodimer in the presence of bound VEGFC or VEGFD. Can also form a heterodimer with KDR. Interacts with PTPN14; the interaction is enhanced by stimulation with VEGFC. Interacts with CRK, GRB2, PTK2/FAK1, SHC1, PIK3R1 and PTPN11/SHP-2. Identified in a complex with SRC and ITGB1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Note=Ligand-mediated autophosphorylation leads to rapid internalization. Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=Ligand-mediated autophosphorylation leads to rapid internalization. Isoform 2: Cell membrane; Single-pass type I membrane protein. Isoform 3: Secreted. Cytoplasm. Tissue Specificity: Detected in endothelial cells (at protein level). Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. Post-translational modifications: Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation in response to H(2)O(2) is mediated by a process that requires SRC and PRKCD activity. Phosphorylation at Tyr-1068 is required for autophosphorylation at additional tyrosine residues. Phosphorylation at Tyr-1063 and Tyr-1337 is important for interaction with CRK and subsequent activation of MAPK8. Phosphorylation at Tyr-1230, Tyr-1231 and Tyr-1337 is important for interaction with GRB2 and subsequent activation of the AKT1 and MAPK1/ERK2 and/or MAPK3/ERK1 signaling pathways. In response to endothelial cell adhesion onto collagen, can also be phosphorylated in the absence of FLT4 kinase activity by SRC. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 7 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain. SWISS: P35916 Gene ID: 2324 Database links: Entrez Gene: 2324 Human Entrez Gene: 14257 Mouse Entrez Gene: 114110 Rat Omim: 136352 Human SwissProt: P35916 Human SwissProt: P35917 Mouse SwissProt: Q91ZT1 Rat Unigene: 646917 Human Unigene: 3291 Mouse Unigene: 81043 Rat VEGFR3又称FLt4主要在成熟组织的淋巴管endothelial cells上表达,VEGF-R3与淋巴管endothelial cells增殖和迁移有关，有刺激淋巴管新生的作用，目前多用于Tumour转移方面的研究。
Product Picture	Tissue/cell: human gastric carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min Incubation: Anti-VEGFR3 Polyclonal Antibody, Unconjugated(SL2202R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VEGFR3) Polyclonal Antibody, Unconjugated (bSL2202R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green). Cell: (mo)Splenocyte(2% BSA at 4°blocked for 30 minutes.1% Paraformaldehyde fixed for 30 minutes,0.1%TritonX-100 permeablized for 2 minutes). Concentration:1:100. Incubation: 40 minutes. Host/Blank: Splenocytes. Flow cytometric analysis of Rabbit Anti-VEGFR3 antibody (SL2202R)(green) compared with control in the absence of primary antibody (blue) followed by Splenocytes. Secondary antibody: Goat Anti-rabbit IgG/FITC antibody (SL0295G-FITC).

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