Product Name	Cytokeratin 19
Chinese Name	细胞角蛋白19抗体
Alias	40 kDa keratin intermediate filament; CK 19; ck19; CK19; CK-19; KRT19; Cytokeratin19; Cytokeratin-19; k19; k1cs; Keratin 19; Keratin-19; Keratin type I 40 kD; Keratin type i 40kD; Keratin type I cytoskeletal 19; krt19; mgc15366; K1C19_HUMAN; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd.
literatures	Specific References  (10)     |     SL2190R has been referenced in 10 publications. [IF=8.025] Xinxin Lan. et al. Xanthan gum/oil body-microgel emulsions with enhanced transdermal absorption for accelerating wound healing. INT J BIOL MACROMOL. 2022 Sep;:  IHC, WB ;  Mouse.   PubMed:36126813 [IF=5.062] Liang Kong. et al. A multi-strategy liposome targeting hepatocellular carcinoma cells and stem cells enhances the chemotherapy effect of doxorubicin in hepatocellular carcinoma. J DRUG DELIV SCI TEC. 2023 Jan;:104188  IHC ;  Mouse.   PubMed:10.1016/j.jddst.2023.104188 [IF=4.631] Xiao-Fan Xu. et al. Aspirin Ameliorates Pancreatic Inflammation and Fibrosis by Inhibiting COX-2 Expression in Experimental Chronic Pancreatitis. J INFLAMM RES. 2022 Aug;15:4737-4749  IHC ;  Mouse.   PubMed:36032936 [IF=3.559] Yin X et al. Zinc oxide nanoparticles ameliorate collagen lattice contraction in human tenon fibroblasts. Arch Biochem Biophys. 2019 Jul 15;669:1-10.  ICC ;  Human.   PubMed:31112708 [IF=2.96] Sottnik et al. Osteocytes serve as a progenitor cell of osteosarcoma. (2014) J.Cell.Biochem. 115:1420-9  IHC ;  Mouse.   PubMed:24700678 [IF=2.792] Yan, Wei. et al. Neural, adipocyte and hepatic differentiation potential of primary and secondary hair follicle stem cells isolated from Arbas Cashmere goats. BMC VET RES. 2022 Dec;18(1):1-18  IF, WB ;  Goat.   PubMed:35971123 [IF=2.24] Chen, Xiaodong, et al. "The effect of porcine ADM to improve the burn wound healing." Int J Clin Exp Pathol 6.11 (2013): 2280-2291.  WB ;  Rat.   PubMed:24228089 [IF=1.28] Chen, Jia, et al. "Biological characterization of metanephric mesenchymal stem cells from the Beijing duck." Experimental and Therapeutic Medicine 11.2 (2016): 439-447.  IF(ICC) ;  Other Species.   PubMed:26893628 [IF=1.18] Ma Caiyun. et al. In Vitro Culture and Multipotency Evaluation of Broiler Umbilical Cord Mesenchymal Stem Cells. BRAZ ARCH BIOL TECHN. 2023 Feb;66:  IF ;  Human.   PubMed:10.1590/1678-4324-2023220031 [IF=1.083] Kammergruber E et al. Morphological and immunohistochemical characteristics of the equine corneal epithelium. Vet Ophthalmol. 2019 Feb 14.  IHC ;  Horse.   PubMed:30767359
Research Area	Tumour  Stem cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	cytoplasmic The cell membrane Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Cytokeratin 19.: 101-200/403
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Function: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Subunit: Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein. Tissue Specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. Similarity: Belongs to the intermediate filament family. SWISS: P08727 Gene ID: 3880 Database links: Entrez Gene: 3880 Human Entrez Gene: 16669 Mouse Omim: 148020 Human SwissProt: P08727 Human SwissProt: P19001 Mouse Unigene: 654568 Human Unigene: 439699 Mouse 结构蛋白（Structural Proteins） CK-19细胞角蛋白-19(又称CYFRa21-1)主要用于Tumour方面的研究，近年来大多用于消化系统恶性Tumour的鉴别，有学者认为：CK-19在胰腺Stem cells分化过程中有细胞角蛋白19的表达，把细胞角蛋白19作为胰腺Stem cells的Maker。阳性部位：主要在胞浆。
Product Picture	Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Cytokeratin 19 (SL2190R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Cytokeratin 19) Polyclonal Antibody, Unconjugated (SL2190R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Cytokeratin 19) Polyclonal Antibody, Unconjugated (SL2190R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Cytokeratin 19) Polyclonal Antibody, Unconjugated (SL2190R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cytokeratin 19) polyclonal Antibody, Unconjugated (SL2190R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cytokeratin 19) polyclonal Antibody, Unconjugated (SL2190R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:MCF-7. Primary Antibody (green line): Rabbit Anti-Cytokeratin 19 antibody (SL2190R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:A549. Primary Antibody (green line): Rabbit Anti-Cytokeratin 19 antibody (SL2190R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (blue line): Hela (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃). Primary Antibody (green line): Rabbit Anti-Cytokeratin 19 antibody (SL2190R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test. Blank control(blue): MCF7(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Cytokeratin 19/FITC Conjugated antibody (SL2190R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

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