Product Name	NQO1
Chinese Name	醌氧化还原酶抗体
Alias	Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1_HUMAN; Phylloquinone reductase; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1; Quinone reductase 1.
literatures	Specific References  (22)     |     SL2184R has been referenced in 22 publications. [IF=7.129] Yuan-yuan Wei. et al. Acute liver injury induced by carbon tetrachloride reversal by Gandankang aqueous extracts through nuclear factor erythroid 2-related factor 2 signaling pathway. ECOTOX ENVIRON SAFE. 2023 Feb;251:114527  WB ;  Mouse.   PubMed:36628874 [IF=7.129] Rui Sang. et al. Taraxasterol alleviates aflatoxin B1-induced liver damage in broiler chickens via regulation of oxidative stress, apoptosis and autophagy. ECOTOX ENVIRON SAFE. 2023 Feb;251:114546  WB ;  Chicken.   PubMed:36646010 [IF=6.992] Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 Apr;:  WB ;  Rat.   PubMed:10.1016/j.jare.2021.04.002 [IF=6.953] Jinshan Wu. et al. Protection by Hosta ventricosa polysaccharides against oxidative damage induced by t-BHP in HepG2 cells via the JNK/Nrf2 pathway. Int J Biol Macromol. 2022 May;208:453  WB ;  Human.   PubMed:35339497 [IF=6.291] Xiao Wang. et al. Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis. Ecotox Environ Safe. 2022 Mar;233:113339  WB ;  Chicken.   PubMed:35219255 [IF=6.081] Naoki Yoshida. et al. Relationship between Cognitive Dysfunction and Age-Related Variability in Oxidative Markers in Isolated Mitochondria of Alzheimer’s Disease Transgenic Mouse Brains. Biomedicines. 2022 Feb;10(2):281  WB ;  Mouse.   PubMed:10.3390/biomedicines10020281 [IF=5.97] Zhang et al. Nrf2 antioxidant pathway suppresses Numb-mediated epithelial-mesenchymal transition during pulmonary fibrosis. (2018) Cell.Death.Dis. 9:83  WB ;  Mouse, Rat, Human.   PubMed:29362432 [IF=5.168] Suiyi Liu. et al. Depleted uranium causes renal mitochondrial dysfunction through the ETHE1/Nrf2 pathway. CHEM-BIOL INTERACT. 2023 Feb;372:110356  WB ;  Rat.   PubMed:36681261 [IF=5.076] Zhang Yifanet al. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxid Med Cell Longev . 2020 Jul 9;2020:6428498.  WB ;  Human.   PubMed:32695257 [IF=4.571] Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297  WB ;  Rat.   PubMed:10.1016/j.tox.2022.153297 [IF=4.527] Han C et al. Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via Nrf2 pathway. Ecotoxicol Environ Saf. 2020 Mar 15;191:110239.  WB ;  Chicken.   PubMed:31991393 [IF=3.909] Wang X et al. Sarcodon imbricatus polysaccharides protect against cyclophosphamide-induced immunosuppression via regulating Nrf2-mediated oxidative stress.(2018) Int. J. Biol. Macromol.Dec;120(Pt A):736-744.  WB ;  Mouse.   PubMed:30171947 [IF=3.83] Wang, Peng, et al. "Geraniin exerts cytoprotective effect against cellular oxidative stress by upregulation of Nrf2-mediated antioxidant enzymes expression via PI3K/AKT and ERK1/2 pathway." Biochimica et Biophysica Acta (BBA)-General Subjects (2015).  WB ;  Human.   PubMed:25917210 [IF=3.06] Wang, Huihui. et al. Anti-inflammatory and Antioxidant Effects of Pyrroloquinoline Quinone in L-NAME-Induced Preeclampsia-Like Rat Model. 2021 Sep 20  WB ;  Rat.   PubMed:10.1007/s43032-021-00743-8 [IF=3.056] Xiao Wang. et al. Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis. Environ Sci Pollut R. 2021 Jan;28(1):936-946  WB ;  Chicken.   PubMed:32827115 [IF=2.688] Zhihai Yu. et al. Two contradictory facades of N-acetylcysteine activity towards renal carcinoma cells. J TAIBAH UNIV SCI. 2022;16(1):423-431  WB ;  Human.   PubMed:10.1080/16583655.2022.2070365 [IF=2.571] Tang et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. (2016) Cell.Stress.Chaperones. 21:239-49  WB ;  Rat.   PubMed:26577463 [IF=2.55] Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22.  WB ;  Mouse.   PubMed:28249220 [IF=2.466] Xiangyou Yu. et al. Upregulation of BRD7 protects podocytes against high glucose-induced apoptosis by enhancing Nrf2 in a GSK-3β-dependent manner. TISSUE CELL. 2022 Jun;76:101813  WB ;  Mouse.   PubMed:35550209 [IF=2.4] Zhao, Feng, et al. "Silymarin attenuates paraquat‐induced lung injury via Nrf2‐mediated pathway in vivo and in vitro." Clinical and Experimental Pharmacology and Physiology (2015).  WB ;  Human.   PubMed:26173462 [IF=1.641] Feng Gao. et al. Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27  WB ;  Bovine.   PubMed:33866621 [IF=1.07] Liu, Cui-Zhen, et al. "HBSP attenuates lipopolysaccharide-induced inflammatory response in human renal proximal tubular epithelial cells by induction of Nrf2 via PI3K/Akt pathway." Int J Clin Exp Med 10.1 (2017): 587-597.  WB ;  Human.   PubMed:ISSN:1940-5901/IJCEM0037069
Research Area	Tumour  Signal transduction  Synthesis and Degradation  Cell type markers  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Pig, Horse, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	31kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NQO1: 201-274/274
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Function: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Subunit: Homodimer. Subcellular Location: Cytoplasm. Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. SWISS: P15559 Gene ID: 1728 Database links: Entrez Gene: 610935 Dog Entrez Gene: 1728 Human Entrez Gene: 18104 Mouse Entrez Gene: 100286873 Pig Entrez Gene: 24314 Rat Omim: 125860 Human SwissProt: P15559 Human SwissProt: Q64669 Mouse SwissProt: P05982 Rat Unigene: 406515 Human Unigene: 252 Mouse Unigene: 11234 Rat Synthesis and Degradation（Synthesis and Degradation） 醌氧化还原酶（NQO1）是一种黄素酶，它催化醌双电子还原反应，以减少氧自由基的产生。经研究发现NQO1可能有抗氧化效应，目前多用于帕金森病（PD）和部分Tumour方面的研究。 醌是一种有毒的化合物,能诱发哺乳动物细胞癌变、突变和坏死。
Product Picture	Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: MCF-7 (Human) Cell Lysate at 30 ug Lane 4: Du145 (Human) Cell Lysate at 30 ug Primary: Anti-NQO1 (SL2184R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD Sample: Lane1: Liver(Rat) Lysate at 30 ug Lane2: Brain(Rat) Lysate at 30 ug Primary: Anti-NQO1 (SL2184R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 31kD Observed band size : 30kD Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-NQO1 (SL2184R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD Tissue/cell: mouse colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NQO1 Polyclonal Antibody, Unconjugated(SL2184R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NQO1) Polyclonal Antibody, Unconjugated (SL2184R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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