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Product Name GCNF Chinese Name 生殖The nucleus因子GCNF/维甲酸受体相关睾丸受体抗体 Alias GCNF 1; GCNF; GCNF1; Germ cell nuclear factor; hGCNF; hRTR; NR 61; NR61; NR6A1; NR6A1_HUMAN; Nuclear receptor subfamily 6 group A member 1; Orphan nuclear receptor NR6A1; Retinoic acid receptor related testis associated receptor; Retinoid receptor related testis specific receptor; Retinoid receptor-related testis-specific receptor; RTR. Research Area Cell biology Developmental biology Neurobiology Signal transduction Stem cells transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 54kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GCNF : 91-190/480 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Germ cell nuclear factor (GCNF) is an orphan member of the nuclear receptor gene superfamily that influences neurogenesis and germ cell development. GCNF can homodimerize and bind DNA. GCNF regulates paracrine interaction between the oocyte and somatic cells by regulating the expression of BMP-15 and GDF-9, to affect female fertility. GCNF is present in spermatocytes and round spermatids of adult male mouse testis; northern blot and ribonuclease protection assays have shown GCNF is predominant in the testis. The gene expresses three alternatively spliced transcript variants.
Function:
Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.
Subunit:
Homodimer. Interacts with UIMC1
Subcellular Location:
Nucleus.
Tissue Specificity:
Shows highest expression in the germ cells of the adult testis.
Similarity:
Belongs to the nuclear hormone receptor family. NR6 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
SWISS:
Q15406
Gene ID:
2649
Database links:
Entrez Gene: 2649 HumanEntrez Gene: 14536 Mouse
Omim: 602778 Human
SwissProt: Q15406 Human
SwissProt: Q64249 Mouse
Unigene: 586460 Human
Unigene: 439703 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCNF) Polyclonal Antibody, Unconjugated (SL21578R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCNF) Polyclonal Antibody, Unconjugated (SL21578R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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