Product Name	PARP1
Chinese Name	多腺苷二磷酸多聚酶抗体(N端)
Alias	ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN.
literatures	Specific References  (19)     |     SL2138R has been referenced in 19 publications. [IF=12.88] Ma, Juan, et al. "A Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals." ACS nano (2015).  WB ;  Mouse.   PubMed:26389709 [IF=11.508] Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12  WB ;  Mouse.   PubMed:33431863 [IF=6.317] Nanqing Jing. et al. Both live and heat-killed Bifidobacterium animalis J-12 alleviated oral ulcers in LVG golden Syrian hamsters by gavage by directly intervening in the intestinal flora structure. FOOD FUNCT. 2023 Jan;:  IHC ;  Hamster.   PubMed:36723265 [IF=4.925] Yuan P et al. The nanomaterial-induced bystander effects reprogrammed macrophage immune function and metabolic profile. Nanotoxicology . 2020 Oct;14(8):1137-1155.  WB ;  Human.   PubMed:32916084 [IF=4.522] Han S et al. FHL1 regulates myoblast differentiation and autophagy through its interaction with LC3. J Cell Physiol. 2019 Oct 21.  WB ;  Chicken.   PubMed:31637727 [IF=4.432] Luya Pu. et al. Icariin arrests cell cycle progression and induces cell apoptosis through the mitochondrial pathway in human fibroblast-like synoviocytes. Eur J Pharmacol. 2021 Dec;912:174585  WB ;  Human.   PubMed:34678240 [IF=3.571] Nandi S et al. Characterization and inception of a triterpenoid astrakurkurol, as a cytotoxic molecule on human hepatocellular carcinoma cells, Hep3B. J Agric Food Chem. 2019 Jul 10;67(27):7660-7673.  WB ;  Human.   PubMed:31250646 [IF=3.367] Lijin Guo. et al. Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis. Front Physiol. 2020; 11: 573676  WB ;  Chicken.   PubMed:33192575 [IF=3.15] Gong H et al. EZH2 inhibitors reverse resistance to gefitinib in primary EGFR wild-type lung cancer cellsBMC Cancer.2020 Dec 4;20(1):1189.  WB ;  Human.   PubMed:33276757 [IF=3.098] Fan Y et al. S5, a Withanolide Isolated from Physalis Pubescens L., Induces G2/M Cell Cycle Arrest via the EGFR/P38 Pathway in Human Melanoma A375 Cells.(2018) Molecules 23(12)  WB ;  Human.   PubMed:30513793 [IF=2.842] Chen J et al. Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats. Diabetes Metab Syndr Obes . 2020 May 20;13:1725-1731.  IHC-P ;  Rat.   PubMed:32547134 [IF=2.72] Sudeshna Nandi. et al. Anti-cancer effect of astrakurkurol from a folklore tribal mushroom on human hepatocellular carcinoma cells via mediating cell cycle inhibition, apoptosis, and migration. 2021 Nov 22  WB ;  Human.   PubMed:34811765 [IF=2.688] Zhihai Yu. et al. Two contradictory facades of N-acetylcysteine activity towards renal carcinoma cells. J TAIBAH UNIV SCI. 2022;16(1):423-431  WB ;  Human.   PubMed:10.1080/16583655.2022.2070365 [IF=2.466] Jinjiang Yang. et al. Platelet-rich plasma attenuates interleukin-1β-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2α. Tissue Cell. 2021 Sep;:101646  WB ;  rat.   PubMed:34536814 [IF=1.984] Wei J et al. Jiawei Foshou San Induces Apoptosis in Ectopic Endometrium Based on Systems Pharmacology, Molecular Docking, and Experimental Evidence. Evid Based Complement Alternat Med. 2019 Oct 27;2019:2360367.  WB ;  Rat.   PubMed:31781263 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.  WB ;  Rat.   PubMed:27665780 [IF=1.45] Omar, S. S., R. G. Aly, and N. M. Badae. "Vitamin E improves testicular damage in streptozocin‐induced diabetic rats, via increasing vascular endothelial growth factor and poly (ADP‐ribose) polymerase‐1." Andrologia (2017).  IHC-P ;  Rat.   PubMed:29164711 [IF=1.445] Li YH et al. Neuroprotective Effect of Fructus broussonetiae on APP/PS1 Mice via Upregulation of AKT/β-Catenin Signaling. Chin J Integr Med. 2020 Jan 4.  WB ;  Mouse&Rat.   PubMed:31903532 [IF=0] Zhou et al. Azithromycin synergistically enhances anti-proliferative activity of vincristine in cervical and gastric cancer cells. (2012) Cancers.(Basel). 4:1318-32  WB ;  Human.   PubMed:24213508
Research Area	Chromatin and nuclear signals  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	111kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PARP: 201-300/1014
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]. Function: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Subunit: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Subcellular Location: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus. Post-translational modifications: Phosphorylated by PRKDC and TXK. Phosphorylated upon DNA damage, probably by ATM or ATR. Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. S-nitrosylated, leading to inhibit transcription regulation activity. Similarity: Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 2 PARP-type zinc fingers. SWISS: P09874 Gene ID: 142 Database links: Entrez Gene: 142 Human Entrez Gene: 11545 Mouse Entrez Gene: 25591 Rat Omim: 173870 Human SwissProt: P09874 Human SwissProt: P11103 Mouse SwissProt: P27008 Rat Unigene: 177766 Human Unigene: 277779 Mouse Unigene: 11327 Rat PARP(poly ADP-ribose polymerase)是DNA修复酶。 PARP是Apoptosis核心成员半胱胺酸蛋白酶(caspase)的切割底物。因此,它在DNA损伤修复与Apoptosis中发挥着重要作用。Anti-PARP p85 是特意的PARPp85片段的特异抗体，由caspase剪切116kDa完整分子而得到的。 PARP是存在于多数真核细胞中的一个多功能蛋白质翻译后修饰酶。它通过识别结构损伤的DNA片段而被激活，对聚腺苷二磷酸核糖聚合酶PARP被认为是DNA损伤的感受器。它还能对许多核蛋白进行聚腺苷二磷酸核糖基化。因此，在DNA损伤修复与Apoptosis中发挥着重要作用，端锚聚合酶在癌细胞端粒结构的调控机制中有重要作用。
Product Picture	Sample: Raji Cell (Human) Lysate at 30 ug Primary: Anti- PARP (SL2138R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD Sample: Hela Cell (Human) Lysate at 30 ug Primary: Anti- PARP (SL2138R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD Sample: Lane 1: 293T (Human) Cell Lysate at 30 ug Lane 2: HL60 (Human) Cell Lysate at 30 ug Lane 3: K562 (Human) Cell Lysate at 30 ug Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 5: U251 (Human) Cell Lysate at 30 ug Lane 6: Hela (Human) Cell Lysate at 30 ug Primary: Anti-PARP (SL2138R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD Sample: Hela(Human) Cell Lysate at 30 ug Raji(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- PARP (SL2138R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD Protein:HepG2 lysate at 30ug; Primary: Anti-PARP (SL2138R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size:111 kD Observed band size:111 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (SL2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse intestines); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (SL2138R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): HL60 cells (blue). Primary Antibody (green line): Rabbit Anti- PARP antibody (SL2138R) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:293T. Primary Antibody (green line): Rabbit Anti-PARP1 antibody (SL2138R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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