Drug Names

Generic Name: Human Insulin Autoantibody (IAA) ELISA Kit

Purpose

Our Human Insulin Autoantibody (IAA) ELISA kit is to assay IAA levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to IAA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen . Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Insulin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IAA and HRP conjugated Insulin antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IAA. You can calculate the concentration of IAA in the samples by comparing the OD of the samples to the standard curve.

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