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Product Name PAN3 Chinese Name PAN3蛋白抗体 Alias hPan3; PAB dependent poly(A) specific ribonuclease subunit 3; PAB-dependent poly(A)-specific ribonuclease subunit 3; PAB1P dependent poly(A) nuclease; PABP dependent poly(A) nuclease 3; PABP1 dependent poly A specific ribonuclease subunit PAN3; Pan 3; PAN deadenylation complex subunit 3; PAN3; PAN3 poly(A) specific ribonuclease subunit; PAN3 poly(A) specific ribonuclease subunit homolog; PAN3 polyA specific ribonuclease subunit homolog; PAN3_HUMAN. Research Area Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 96kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PAN3: 541-640/887 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail PAN3 (PAN3 Poly(A) Specific Ribonuclease Subunit) is a Protein Coding gene. Among its related pathways are Deadenylation-dependent mRNA decay and Gene Expression. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and poly(A)-specific ribonuclease activity.
Function:
Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3-5 exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5-3 exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with PABP and to miRNA targets via its interaction with GW182 family proteins.
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
SWISS:
Q58A45
Gene ID:
255967
Database links:Entrez Gene: 255967 Human
Entrez Gene: 72587 Mouse
SwissProt: Q58A45 Human
SwissProt: Q640Q5 Mouse
Unigene: 645015 Human
Unigene: 320469 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAN3) Polyclonal Antibody, Unconjugated (SL21013R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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