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Product Name PAF49 Chinese Name RNA聚合酶II相关因子PAF49抗体 Alias A34.5; Anti sense to ERCC 1 protein; Antisense to ERCC-1 protein; ASE 1; ASE-1; ASE1; CAST; CD3 epsilon associated protein; CD3-epsilon-associated protein; CD3E antigen, epsilon polypeptide associated protein; CD3E associated protein; CD3e molecule, epsilon associated protein; CD3E-associated protein; CD3EAP; DNA directed RNA polymerase I subunit RPA34; DNA-directed RNA polymerase I subunit RPA34; MGC118851; PAF 49; RNA polymerase I associated factor PAF49; RNA polymerase I-associated factor PAF49; RPA34_HUMAN. Research Area Cell biology transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PAF49: 411-510/510 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail CD3EAP (CD3e Molecule Associated Protein) is a Protein Coding gene. Among its related pathways are RNA Polymerase I Promoter Escape and Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3. GO annotations related to this gene include poly(A) RNA binding and DNA-directed 5-3 RNA polymerase activity.
Function:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.Component of RNA polymerase I which synthesizes ribosomal RNA precursors.
Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
Subcellular Location:
Nucleus > nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
Post-translational modifications:
Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.
Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the eukaryotic RPA34 RNA polymerase subunit family.
SWISS:
O15446
Gene ID:
10849
Database links:Entrez Gene: 10849 Human
Omim: 107325 Human
SwissProt: O15446 Human
Unigene: 710495 Human
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