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Product Name GPR49/LGR5 Chinese Name G protein-coupled receptor49抗体 Alias G protein coupled receptor 49; G-protein coupled receptor 49; G-protein coupled receptor 67; GPCR GPR49; G protein coupled receptor 67; FEX; g protein-coupled receptor fex; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR49; GPR67; Leucine rich repeat containing G protein coupled receptor 5; LGR5; HG 38; HG38; LGR5; LGR5_HUMAN; MGC117008; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; Orphan G protein coupled receptor HG38. literatures Specific References (1) | SL20746R has been referenced in 1 publications.[IF=2.792] Yan, Wei. et al. Neural, adipocyte and hepatic differentiation potential of primary and secondary hair follicle stem cells isolated from Arbas Cashmere goats. BMC VET RES. 2022 Dec;18(1):1-18 IF, WB ; Goat.Research Area Tumour Signal transduction Stem cells Apoptosis G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Pig, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 98kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GPR49/LGR5: 331-430/907 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Function:
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Subunit:
Identified in a complex composed of RNF43, LGR5 and RSPO1.
Subcellular Location:
Cell membrane; Multi-pass membrane protein
Tissue Specificity:
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.
SWISS:
O75473
Gene ID:
8549
Database links:Entrez Gene: 8549 Human
Entrez Gene: 14160 Mouse
Omim: 606667 Human
SwissProt: O75473 Human
SwissProt: Q9Z1P4 Mouse
Unigene: 658889 Human
Unigene: 42103 Mouse
Unigene: 214063 Rat
Lgr5基因(Wnt细胞信号系统)是一种G protein-coupled receptor,已经被确定为几种成年组织和癌症中Stem cells的一个独特标记。Lgr5最初是在结肠癌细胞中发现的,有报道称:在恶化前的小鼠腺瘤中也有发现,这说明Lgr5很可能也是其他组织成体Stem cells和癌症Stem cells的Maker。Product Picture Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-GPR49/LGR5 (SL20746R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100 kD
Observed band size: 105 kD
Sample:
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti-LGR5 (SL20476R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 115 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (SL20746R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR49 LGR5) Polyclonal Antibody, Unconjugated (SL20746R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR49 LGR5) Polyclonal Antibody, Unconjugated (SL20746R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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