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Product Name HGF beta Chinese Name 肝细胞生长因子β抗体 Alias HGF-beta; HGFB; F TCF; Hepatocyte growth factor; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor precursor; Hepatopoietin A; HPTA; Lung fibroblast derived mitogen; Scatter factor; SF; hepatocyte growth factor beta-chain; Hepatocyte growth factor beta chain; HGF_HUMAN. Research Area Tumour Signal transduction Growth factors and hormones Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 26/80kDa Cellular localization The cell membrane Extracellular matrix Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HGF beta: 501-600/723 Lsotype IgG Purification affinity purified by Protein A Buffer Solution Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the lasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Function:
HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity.
Subunit:
Dimer of an alpha chain and a beta chain linked by a disulfide bond.
DISEASE:
Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39) [MIM:608265]. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Similarity:
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
SWISS:
P14210
Gene ID:
3082
Database links:Entrez Gene: 3082 Human
Entrez Gene: 15234 Mouse
Omim: 142409 Human
SwissProt: P14210 Human
SwissProt: Q08048 Mouse
Unigene: 396530 Human
Unigene: 267078 Mouse
Unigene: 10468 Rat
Product Picture Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-HGF beta (SL20688R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26/80 kD
Observed band size: 26/80 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-HGF beta antibody (SL20688R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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