Product Name	GPR30
Chinese Name	G protein-coupled receptor30抗体
Alias	G Protein Coupled Receptor 30; G-protein coupled receptor 30; G-protein coupled estrogen receptor 1; Membrane estrogen receptor; mER; Chemokine receptor-like 2; IL8-related receptor DRY12; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; Lymphocyte-derived G-protein coupled receptor; LYGPR; GPCR-BR; CEPR; CMKRL2; DRY12; GPER.
literatures	Specific References  (1)     |     SL20643R has been referenced in 1 publications. [IF=1.487]  Chengjian Zhou . et al. Daidzein stimulates fatty acid-induced fat deposition in C2C12 myoblast cells via the G protein-coupled receptor 30 pathway. 2020 Nov 08  WB,IF ;  Mouse.   PubMed:33164657
Research Area	Cell biology  Neurobiology  Signal transduction  The cell membrane受体  G protein-coupled receptor  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	42kDa
Cellular localization	The nucleus cytoplasmic The cell membrane Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GPR30: 251-350/375 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	G protein-coupled receptors (GPRs, or GPCRs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GRP30 is a member of this family and is an orphan receptor. GPR30 expression has been reported in brain, breast carcinoma, blood, bone marrow, CNS, heart, liver, lung, lymph node, placenta, and spleen. In brain, GPR30 is expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. However, unlike other known G protein-coupled receptors, GPR30 localizes to the endoplasmic reticulum, where it specifically binds estrogen and estrogen derivatives. Function: Receptor for estrogen. Subcellular Location: Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor. Tissue Specificity: Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q99527 Gene ID: 2852 Database links: Entrez Gene: 2852 Human Entrez Gene: 76854 Mouse Entrez Gene: 171104 Rat Omim: 601805 Human SwissProt: Q99527 Human SwissProt: Q8BMP4 Mouse SwissProt: O08878 Rat Unigene: 20961 Human Unigene: 389706 Mouse Unigene: 9806 Rat GPR30是一种膜性雌激素蛋白受体又称非基因型雌激素膜性受体GPR30。GPR30受体广泛表达于海马、下丘脑、子宫、卵巢、乳腺、骨和Cardiovascular等全身多个系统、器官和组织,在细胞内主要定位于The cell membrane、内质网、Mitochondrion和高尔基体。 GPER与雌激素结合, ERK、PI3K/AKT)、 cAMP等第二信使途径发挥快速非基因效应,在神经系统、生殖系统、运动系统和Cardiovascular系统中发挥重要的生理作用。
Product Picture	Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-GPR30 (SL20643R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kD Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-GPR30 (SL20643R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kD Sample: MCF-7 (Human) Lysate at 30 ug Primary: Anti-GPR30 (SL20643R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kD Sample: 293T (Human) Lysate at 30 ug Primary: Anti-GPR30 (SL20643R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kD Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Rat) Lysate at 40 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 5: MCF-7 (Human) Cell Lysate at 30 ug Lane 6: Heart (Mouse) Lysate at 40 ug Lane 7: Heart (Rat) Lysate at 40 ug Primary: Anti-GPR30 (SL20643R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (SL20643R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (SL20643R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (SL20643R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (SL20643R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (GPR30) polyclonal Antibody, Unconjugated (SL20643R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): A431 cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃). Primary Antibody (green line): Rabbit Anti-GPR30 antibody (SL20643R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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