Product Name	Phospho-delta Opioid Receptor (Ser363)
Chinese Name	磷酸化D型阿片受体抗体
Alias	Delta Opioid Receptor (phospho S363); p-Delta Opioid Receptor (phospho S363); Delta Opioid Receptor (phospho S363); Delta Opioid Receptor (p-S363); Delta Opioid Receptor (phospho Ser363); Delta type opioid receptor; Delta type opioid receptor DOR 1; DOR 1; DOR1; mDOR; Nbor; Opioid receptor delta 1; OPRD; OPRD1; OPRD_HUMAN; Delta-type opioid receptor; D-OR-1; DOR-1.
Product Type	Phosphorylated anti
Research Area	immunology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	40kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human delta Opioid Receptor around the phosphorylation site of Ser363: TP(p-S)DG
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The opioid receptors are G-protein coupled, seven-transmembrane domain receptors for enkephalins, endorphins, and dynorphins. Three different opioid receptor subtypes (kappa , delta, and mu) were first identified by their different selectivities for various naturally occurring alkaloid opioid ligands, and subsequently confirmed by molecular cloning. The amino acid sequences of the opioid receptor subtypes are ~70% homologous, and are similar to somatostatin receptors (SSTRs) showing ~40 % homology with SSTR1. G-protein binding is thought to occur at the third intracellular loop of the opioid receptors, which is also the location of consensus sequences for phosphorylation of the receptor. Interestingly, the genes encoding the specific receptor subtypes are found on different chromosomes in both the human and mouse genomes. Function: Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. receptor for enkephalins. Subunit: Interacts with GPRASP1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P41143 Gene ID: 4985 Database links: Entrez Gene: 4985 Human Entrez Gene: 18386 Mouse Omim: 165195 Human SwissProt: P41143 Human SwissProt: P32300 Mouse Unigene: 372 Human
Product Picture	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Cerebellum tissue lysates Primary: Anti-Phospho-delta Opioid Receptor (Ser363) (SL20197R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 47 kDa Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-delta Opioid Receptor(Ser363) Polyclonal Antibody, Unconjugated (SL20197R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell:human Neurological glioblastoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OPRD Polyclonal Antibody, Unconjugated(SL20197R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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