Product Name	phospho-NFAT2 (Ser233)
Chinese Name	磷酸化活化TThe nucleus因子1蛋白抗体
Alias	NFAT2 (phospho S233); p-NFAT2 (phospho S233); p-NFAT2 (Ser235 mouse) ; NFAT2; MGC138448; NF ATc; NF ATc1; NFAT 2; NFATc1; NFAT transcription complex cytosolic component; NFATC 1; NFATc; NFATc1; Nuclear factor of activated T cells cytoplasmic 1; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1; Nuclear factor of activated T cells cytosolic component 1; NFAC1_HUMAN.
literatures	Specific References  (1)     |     SL19785R has been referenced in 1 publications. [IF=8.109] Ling Wu. et al. Calcium Channel Blocker Nifedipine Suppresses Colorectal Cancer Progression and Immune Escape by Preventing NFAT2 Nuclear Translocation. Cell Rep. 2020 Oct;33:108327  IF,IHC ;  Human.   PubMed:33113363
Product Type	Phosphorylated anti
Research Area	Cell biology  Neurobiology  Apoptosis  transcriptional regulatory factor  t-lymphocyte  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Pig, Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	101kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human NFAT2 around the phosphorylation site of Ser233: LG(p-S)SP
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. The biological function of this helicase has not yet been determined. Two alternatively spliced transcripts, which encode the same isoform but differ in their 5' and 3' UTRs, have been described. [provided by RefSeq, Jul 2008] Function: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. Tissue Specificity: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Post-translational modifications: Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin. Similarity: Contains 1 RHD (Rel-like) domain. SWISS: O95644 Gene ID: 4772 Database links: Entrez Gene: 4772 Human Omim: 600489 Human SwissProt: O95644 Human Unigene: 534074 Human Unigene: 701518 Human
Product Picture	Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-phospho-NFAT2 (Ser233) (Ser235 mouse)(SL19785R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 101 kD Observed band size: 101 kD Paraformaldehyde-fixed, paraffin embedded (Rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-NFAT2 (Ser233) (Ser235 mouse)) Polyclonal Antibody, Unconjugated (SL19785R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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