Product Name	RBM17
Chinese Name	RNABinding protein17抗体
Alias	45 kDa-splicing factor; RBM 17; Rbm17; RNA binding motif protein 17; RNA binding motif protein17; RNA-binding motif protein 17; SPF 45; SPF45; SPF45_HUMAN; Splicing factor (45 kD); Splicing factor (45kD); Splicing factor 45; Splicing factor 45 kDa; Splicing factor 45kDa.
Research Area	Cell biology  Neurobiology  Binding protein  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RBM17: 231-330/401
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. [provided by RefSeq, Mar 2009] Function: Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. Subcellular Location: Nucleus. Similarity: Contains 1 G-patch domain. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q96I25 Gene ID: 84991 Database links: Entrez Gene: 84991 Human Entrez Gene: 76938 Mouse Entrez Gene: 291295 Rat Omim: 606935 Human SwissProt: Q96I25 Human SwissProt: Q8JZX4 Mouse Unigene: 498548 Human Unigene: 182769 Mouse Unigene: 20694 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM17) Polyclonal Antibody, Unconjugated (SL19765R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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