Product Name	RBM14
Chinese Name	RNABinding protein14抗体
Alias	CoAA; Coactivator Activator; RBM 14; RBM14; RNA binding motif protein 14; RNA binding protein 14; RRM containing coactivator activator/modulator; SIP; Synaptotagmin interacting protein; SYT interacting protein 1; SYT interacting protein; SYT interacting protein SIP; SYTIP 1; SYTIP1.
Research Area	Cell biology  Signal transduction  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	69kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RBM14: 101-200/669
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene. [provided by RefSeq, Oct 2011] Function: Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1. Subunit: Isoform 1 interacts with NCOA6, CITED1 and XRCC5/KU86. Isoform 1 interacts with SS18 isoform 1. Isoform 1 interacts with SS18 isoform 2. Subcellular Location: Nuclear Tissue Specificity: Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. Similarity: Contains 2 RRM (RNA recognition motif) domains. SWISS: Q96PK6 Gene ID: 10432 Database links: Entrez Gene: 511512 Cow Entrez Gene: 10432 Human Entrez Gene: 56275 Mouse Entrez Gene: 170900 Rat Omim: 612409 Human SwissProt: Q5EA36 Cow SwissProt: Q96PK6 Human SwissProt: Q8C2Q3 Mouse Unigene: 533712 Human Unigene: 8556 Rat
Product Picture	Sample: Hela(Human) Cell Lysate at 40 ug 293T(Human) Cell Lysate at 40 ug Primary: Anti-RBM14 (SL19763R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 69 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM14) Polyclonal Antibody, Unconjugated (SL19763R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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