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Product Name OLFML2B Chinese Name 嗅介蛋白样蛋白2B抗体 Alias Olfactomedin like 2B; Olfactomedin like protein 2B; Olfactomedin-like protein 2B; OLFML2B; OLM2B_HUMAN; Photomedin 2; Photomedin-2; RP11-227F8.1. literatures Specific References (1) | SL19640R has been referenced in 1 publications.[IF=3.288] Liu J et al. Bioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing tools.BMC Cancer. 2019 Mar 13;19(1):227. IHC-P ; Human.Research Area Cell biology Neurobiology Stem cells Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 84kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human OLFML2B: 451-550/750 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Sample:
Eye (Mouse) Lysate at 40 ug
Epithelium (Mouse) Lysate at 40 ug
Primary: Anti-OLFML2B(SL19640R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84kD
Observed band size: 84kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OLFML2B) Polyclonal Antibody, Unconjugated (SL19640R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OLFML2B) Polyclonal Antibody, Unconjugated (SL19640R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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