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Product Name NT5DC2 Chinese Name NT5DC2蛋白抗体 Alias 5' nucleotidase domain containing 2; 5'-nucleotidase domain-containing protein 2; FLJ12442; NT5D2_HUMAN; Nt5dc2. Research Area Cell biology Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 61kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NT5DC2: 251-350/520 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail NT5DC2 (5'-Nucleotidase Domain Containing 2) is a Protein Coding gene. GO annotations related to this gene include hydrolase activity and 5-nucleotidase activity. An important paralog of this gene is NT5DC3.
Similarity:
Belongs to the 5'(3')-deoxyribonucleotidase family.
SWISS:
Q9H857
Gene ID:
64943
Database links:Entrez Gene: 64943 Human
SwissProt: Q9H857 Human
Unigene: 84753 Human
Unigene: 2956 Rat
Product Picture Sample:
HT1080(Human) Cell Lysate at 30 ug
Primary: Anti-NT5DC2 (SL19491R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NT5DC2) Polyclonal Antibody, Unconjugated (SL19491R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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