TEL: +86 571 56623320 EMAIL: [email protected]
Product Name NRAMP1 Chinese Name 自然抗性相关巨噬细胞蛋白1抗体 Alias LSH; Natural resistance associated macrophage protein 1; Natural resistance-associated macrophage protein 1; NRAM1_HUMAN; NRAMP 1; NRAMP; PBC; SLC11A1; Solute carrier family 11 (proton coupled divalent metal ion transporters) member 1; solute carrier family 11 (sodium/phosphate symporters) member 1; Solute carrier family 11 member 1. Research Area Tumour Cell biology immunology Signal transduction The cell membrane蛋白 Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Cow, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 60kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NRAMP1: 371-470/550 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
Function:
Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.
Subcellular Location:
Membrane.
Tissue Specificity:
Macrophages; peripheral blood leukocytes, lung, spleen and liver.
Similarity:
Belongs to the NRAMP family.
SWISS:
P49279
Gene ID:
6556
Database links:Entrez Gene: 6556 Human
Entrez Gene: 18173 Mouse
Omim: 600266 Human
SwissProt: P49279 Human
SwissProt: P41251 Mouse
Unigene: 591607 Human
Unigene: 2913 Mouse
Unigene: 105919 Rat
Product Picture Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NRAMP1 Polyclonal Antibody, Unconjugated(SL19341R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Scan Wechat Qrcode