Product Name	NOL4
Chinese Name	核仁蛋白4抗体
Alias	HRIHFB2255; NOL 4; NOL4; NOL4_HUMAN; NOLP; Nucleolar localized protein; Nucleolar protein 4; Nucleolar-localized protein.
Research Area	Cell biology  Chromatin and nuclear signals  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Cow, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	72kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NOL4: 1-100/638
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	NOL4 is a 524 amino acid protein that is predominantly expressed in testis and brain. NOL4 contains at least two domains that direct it to subnuclear locations. The gene encoding NOL4 is located on human chromosome 18, which houses over 300 protein-coding genes and contains nearly 76 million bases. There are a variety of diseases associated with defects in chromosome 18-localized genes, some of which include Trisomy 18 (also known as Edwards syndrome), Niemann-Pick disease, hereditary hemorrhagic telangiectasia, erythropoietic protoporphyria and follicular lymphomas. There are two isoforms of NOL4 that are produced as a result of alternative splicing events. Subcellular Location: Nucleus > nucleolus. Tissue Specificity: Expressed predominantly in fetal brain, adult brain and testis. SWISS: O94818 Gene ID: 8715 Database links: Entrez Gene: 8715 Human Omim: 603577 Human SwissProt: O94818 Human Unigene: 514795 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NOL4) Polyclonal Antibody, Unconjugated (SL19310R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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