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Product Name NPTII/Neomycin Phosphotransferase 2 Chinese Name 肺炎克雷伯菌新霉素磷酸转移酶2抗体 Alias Klebsiella pneumoniae Aminoglycoside 3' phosphotransferase; APH(3') II; APH(3')II; aphA; EC 2.7.1.95; Kan; Kanamycin kinase type II; Kanamycin kinase, type VIII; kanamycin resistance gene; Neo; Neomycin kanamycin phosphotransferase type II; Neomycin kanamycin phosphotransferase type VIII; Neomycin phosphotransferase II; Neomycine phosphotransferase; NPT II; NPTII; KKA2_KLEPN. Research Area Tumour Cell biology Bacteria and viruses The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 32kDa Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Klebsiella pneumoniae Neomycin Phosphotransferase 2: 101-200/264 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin? G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
Similarity:
Belongs to the aminoglycoside phosphotransferase family.
SWISS:
P00552
Gene ID:
N/A
Database links:
SwissProt: P00552 Klebsiella pneumoniae
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