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Product Name phospho-MYL12B (Ser20) Chinese Name 磷酸化肌球蛋白轻链多肽4抗体 Alias Mylc2b (phospho S20); p-b (phospho S20);ML12B_HUMAN; MLC-2; MLC-2a; MLC-B; MLC20; MRLC2; MYL12B; MYLC2B; Myosin light chain 12B regulatory; Myosin regulatory light chain 12B; myosin regulatory light chain 2; Myosin regulatory light chain 2-B; myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; Myosin regulatory light chain MRLC2; myosin, light chain 12B, regulatory; OTTHUMP00000162244; OTTHUMP00000165806; OTTHUMP00000165807; OTTHUMP00000165808; SHUJUN-1; smooth muscle isoform. Product Type Phosphorylated anti Research Area Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human Mylc2b around the phosphorylation site of Ser20: AT(p-S)NV Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008]
Function:
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
Tissue Specificity:
Ubiquitously expressed in various hematopoietic cells.
Post-translational modifications:
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
Similarity:
Contains 3 EF-hand domains.
SWISS:
P24844
Gene ID:
10398
Database links:
Entrez Gene: 10398 Human
Entrez Gene: 98932 Mouse
Omim: 609905 Human
SwissProt: P20689 Human
SwissProt: P24844 Human
SwissProt: Q9CQ19 Mouse
Unigene: 504687 Human
Unigene: 271770 Mouse
Unigene: 228729 Rat
Unigene: 6870 Rat
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