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Product Name ZFP38 Chinese Name Zinc finger protein38抗体 Alias DKFZp434L134; DKFZp686H10254; KOX25; NY REN 21; NYREN21; Renal carcinoma antigen NY REN 21; Renal carcinoma antigen NY-REN-21; Zfp-38; ZFP38; zinc finger and SCAN domain containing 21; Zinc finger and SCAN domain containing protein 21; Zinc finger and SCAN domain-containing protein 21; zinc finger protein 38 (KOX 25); Zinc finger protein 38 homolog; zinc finger protein NY REN 21 antigen; Zipro1; ZNF38; ZSC21_HUMAN; ZSCAN21. Research Area Cell biology Developmental biology transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 54kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ZFP38: 351-450/473 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Sample:
Testis (Mouse) Lysate at 40 ug
Testis (Rat) Lysate at 40 ug
Primary: Anti-ZFP38 (SL19126R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFP38) Polyclonal Antibody, Unconjugated (SL19126R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFP38) Polyclonal Antibody, Unconjugated (SL19126R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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