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Product Name MetAP1 Chinese Name 氨基肽酶1抗体 Alias Methionyl Aminopeptidase 1; AMPM1_HUMAN; DKFZp781C0419; KIAA0094; MAP 1; MAP1A; MetAP-1; MetAP1; metap1; MetAP1A; Methionine aminopeptidase 1; Peptidase M 1. Research Area Tumour Cell biology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Dog, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 43kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MetAP1: 51-150/386 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail MetAP-1 is a 394-amino acid protein that is expressed at low levels in all tissues, but is highly expressed in skeletal muscles. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. The control of cell proliferation in mammalian cells is directly linked and strictly dependent on the evolutionarily highly conserved mechanism that MetAP-1 employs. Eukaryotes contain both MetAP-1 and MetAP-2, whereas prokaryotes possess only the MetAP-1 enzyme. Pyridine-2-carboxylic acid thiazol-2-ylamide (PCAT) forms a scaffold that inhibits the action of MetAP-1, while 1,2,4-triazol is a non-peptide inhibitor of MetAP-1 binding to the active site with the N1 and N2 atoms of the triazole moiety complexing two divalent ions.
Function:
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle.
Subunit:
Associates with the 60S ribosomal subunit of the 80S translational complex.
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the peptidase M24A family.
Methionine aminopeptidase type 1 subfamily.
SWISS:
P53582
Gene ID:
23173
Database links:Entrez Gene: 23173 Human
Entrez Gene: 75624 Mouse
Omim: 610151 Human
SwissProt: P53582 Human
SwissProt: Q8BP48 Mouse
Unigene: 480364 Human
Unigene: 26833 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MetAP1) Polyclonal Antibody, Unconjugated (SL18806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MetAP1) Polyclonal Antibody, Unconjugated (SL18806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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