Product Name	Peroxiredoxin 3
Chinese Name	硫氧还蛋白还原酶3抗体
Alias	Antioxidant protein 1; AOP 1; AOP-1; AOP1; HBC189; MER5; MGC104387; MGC24293; mitochondrial; Peroxiredoxin III; Peroxiredoxin-3; PRDX3; PRDX3_HUMAN; PRO1748; Protein MER5 homolog; PRX III; Prx-III; PRX3; SP 22; SP-22; SP22; Thioredoxin dependent peroxide reductase mitochondrial; Thioredoxin-dependent peroxide reductase.
literatures	Specific References  (1)     |     SL1874R has been referenced in 1 publications. [IF=2.04] Gao, Hong, et al. "Comparative study of Hsp27, GSK3β, Wnt1 and PRDX3 in Hirschsprungs disease." International Journal of Experimental Pathology (2014).  WB ;  Human.   PubMed:24773279
Research Area	Tumour  Chromatin and nuclear signals  Kinases and Phosphatases  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	21kDa
Cellular localization	cytoplasmic Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PRDX3: 161-256/256
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The Peroxiredoxin (Prdx, Prx, or Trx-Px) family of enzymes is a recently identified family of peroxidases found in free-living organisms. The six known isoforms (Prx1-6) play an important role in protecting lipids, enzymes and DNA against peroxides, such as hydrogen peroxide. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. This is acomplished by the active cysteine of Prx reducing peroxides, which is then converted into a transient cysteine sulfenic acid or cysteine sulfinic acid. If Prx protection is inadequate against peroxidases, the resulting protein and DNA damage may result in neurological disease such as Alzheimer's or DNA damage leading to cancer. Function: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Subunit: Dodecameric ring assembled from homodimeric units; disulfide-linked, upon oxidation. The rings have an approximate diameter of 150 A and a central hole of 70 A. 3-5% of the rings are interlocked by pairs. Binds MAP3K13. Interacts with NEK6. Interacts with LRRK2. Subcellular Location: Mitochondrion. Post-translational modifications: Phosphorylated by LRRK2; phosphorylation reduces perodixase activity. Similarity: Belongs to the AhpC/TSA family. Contains 1 thioredoxin domain. SWISS: P30048 Gene ID: 10935 Database links: Entrez Gene: 281998 Cow Entrez Gene: 10935 Human Entrez Gene: 11757 Mouse Entrez Gene: 64371 Rat Omim: 604769 Human SwissProt: P35705 Cow SwissProt: P30048 Human SwissProt: P20108 Mouse SwissProt: Q9Z0V6 Rat Unigene: 523302 Human Unigene: 29821 Mouse Unigene: 2011 Rat
Product Picture	Protein: brain(rat) lysates at 30ug; heart(rat) lysates at 30ug; Primary: Anti-PRDX3 (SL1874R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 20kD,28kD Observed band size : 22kD Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peroxiredoxin 3) Polyclonal Antibody, Unconjugated (SL1874R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peroxiredoxin 3) Polyclonal Antibody, Unconjugated (SL1874R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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