TEL: +86 571 56623320 EMAIL: [email protected]
Product Name phospho-MDC1 (Ser964) Chinese Name 磷酸化DNA损伤关卡蛋白1抗体 Alias MDC1 (phospho S964); p-MDC1 (phospho S964); DKFZp781A0122; Homologue to Drosophila photoreceptor protein calphotin; KIAA0170; KIAA0170; MDC 1; Mdc1; MDC1_HUMAN; Mediation of DNA damage checkpoint 1; Mediator of DNA damage checkpoint 1; Mediator of DNA Damage Checkpoint Protein 1; Mediator of DNA Damage Checkpoint Protein 1; NFBD 1; NFBD1; NFBD1; Nuclear factor with BRCT domains 1; Nuclear Factor with BRCT Domains Protein 1; Nuclear Factor with BRCT Domains Protein 1. Product Type Phosphorylated anti Research Area Tumour Cell biology Apoptosis transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 227kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human MDC1 around the phosphorylation site of Ser964: AS(p-S)PT Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008]
Function:
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
Subcellular Location:
Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
Tissue Specificity:
Highly expressed in testis.
Post-translational modifications:
Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
Similarity:
Contains 2 BRCT domains.
Contains 1 FHA domain.
SWISS:
Q14676
Gene ID:
9656
Database links:Entrez Gene: 9656 Human
GenBank: NP_055456 Human
Omim: 607593 Human
SwissProt: Q14676 Human
Unigene: 433653 Human
Unigene: 653495 Human
Scan Wechat Qrcode