Product Name	PRCP
Chinese Name	脯氨酰羧肽酶抗体
Alias	prolylcarboxypeptidase; Angiotensinase C; HUMPCP; Lysosomal carboxypeptidase C; Lysosomal Pro x carboxypeptidase; PCP; Proline carboxypeptidase.
Research Area	Kinases and Phosphatases  Endocrinopathy
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	55kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PRCP: 101-200/496
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PRCP belongs to the peptidase S28 family. It cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Function: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Subunit: Homodimer. Subcellular Location: Lysosome. Tissue Specificity: Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney. Similarity: Belongs to the peptidase S28 family. SWISS: P42785 Gene ID: 5547 Database links: Entrez Gene: 5547 Human Omim: 176785 Human SwissProt: P42785 Human Unigene: 523936 Human PRCP蛋白位于下丘脑，其作用是调节人体内黑素细胞刺激素来遏制人的饥饿感，让人保持精力充沛。 用于从而达到减肥目的。
Product Picture	Sample: bone (Mouse) Lysate at 40 ug Primary: Anti-PRCP(SL1873R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRCP) Polyclonal Antibody, Unconjugated (SL1873R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

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