Product Name	PD-1
Chinese Name	程序性死亡1（CD279）抗体
Alias	Programmed cell death protein 1; CD279; CD279 antigen; hPD 1; hPD-1; hSLE1; PD 1; PD1; PDCD 1; PDCD1; PDCD1_HUMAN; Programmed cell death 1; Protein PD 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2.
literatures	Specific References  (13)     |     SL1867R has been referenced in 13 publications. [IF=6.832] Aussel, Clotilde. et al. IL-1β primed mesenchymal stromal cells moderate hemorrhagic shock-induced organ injuries. Stem Cell Res Ther. 2021 Dec;12(1):1-16  FC ;  Rat.   PubMed:34353366 [IF=6.639] Ling Ding. et al. Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity. Cancers. 2021 Jan;13(22):5729  WB ;  Human.   PubMed:34830884 [IF=4.657] He, Xiaojing. et al. Inhibition of PD-1 Alters the SHP1/2-PI3K/Akt Axis to Decrease M1 Polarization of Alveolar Macrophages in Lung Ischemia–Reperfusion Injury. INFLAMMATION. 2022 Nov;:1-16  WB ;  Rat.   PubMed:36357527 [IF=4.068] Li Wang. et al. Radiotherapy upregulated immune checkpoints contribute to the development of second primary OSCC. ORAL DIS. 2023 May;:  WB ;  Rat.   PubMed:37213085 [IF=3.926] Xin YAN. et al. PD-1/PD-L1 inhibition promotes hepatic regeneration in small-for-size liver following extended hepatectomy. CYTOKINE. 2022 Nov;159:156017  IHC, IF ;  Rat.   PubMed:10.1016/j.cyto.2022.156017 [IF=3.376] Mirjana Nacka-Aleksić. et al. Sex as a confounding factor in the effects of ageing on rat lymph node t cell compartment. Exp Gerontol. 2020 Dec;142:111140  FC ;  Rat.   PubMed:33129930 [IF=3.375] Fei Li. et al. POU2F1 induces the immune escape in lung cancer by up-regulating PD-L1. Am J Transl Res. 2021; 13(2): 672–683  other ;   PubMed:33594317 [IF=2.96] Zhang, Song-An, et al. "Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway." Tumor Biology 39.3 (2017): 1010428317692237.  IHC-P ;  Rat.   PubMed:28351328 [IF=2.924] Wang, Shan. et al. Excessive Immune Activation and the Correlation with Decreased Expression of PD-1 at the Maternal–Fetal Interface in Preeclampsia. REPROD SCI. 2022 Jun;:1-11  IHC ;  Human.   PubMed:35708884 [IF=2.885] Sun, Xinxin. et al. 0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats. ODONTOLOGY. 2022 Sep;:1-11  IHC ;  Rat.   PubMed:36057921 [IF=2.48] Szabó, Krisztina, et al. "Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis." Immunology Letters (2017).  IHC-P ;  Human.   PubMed:28431963 [IF=2.199] Yang J et al. Programmed cell death protein 1 promotes hepatitis B virus transmission through the regulation of ERK1/2-mediated trophoblasts differentiation. Arch Gynecol Obstet. 2019 Dec 2.  ICF ;  Human.   PubMed:31792623 [IF=1.808] Asuman Feda Bayrak. et al. Association of immune response with overall and disease-free survival in laryngeal squamous cell carcinomas. AM J OTOLARYNG. 2022 Jul;43:103477  IHC ;  Human.   PubMed:35537231
Research Area	Tumour  Cell biology  immunology  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Detection molecular weight	55-60 kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PD-1: 201-288/288
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020] Function: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors. Subunit: Monomer. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Ta,Ba,Ma,Thy DISEASE: Systemic lupus erythematosus 2 (SLEB2) [MIM:605218]: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. {ECO:0000269|PubMed:12402038}. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q15116 Gene ID: 5133 Database links: Entrez Gene: 5133 Human Entrez Gene: 18566 Mouse Entrez Gene: 301626 Rat Omim: 600244 Human SwissProt: Q15116 Human SwissProt: Q02242 Mouse Unigene: 158297 Human Unigene: 5024 Mouse Unigene: 105023 Rat
Product Picture	Sample: Raji(Human) Cell Lysate at 30 ug Thymus(Mouse) Lysate at 40 ug Lymph node(Mouse) Lysate at 40 ug Spleen(Mouse) Lysate at 40 ug Raw264.7(Mouse) Cell Lysate at 40 ug Thymus (Rat) Lysate at 40 ug Primary: Anti-PD-1 (SL1867R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 58 kD Sample: Hela(Human) Cell Lysate at 30 ug MOLT-4(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-PD-1 (SL1867R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 55 kD Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PD-1 ) Polyclonal Antibody, Unconjugated (SL1867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PD-1 ) Polyclonal Antibody, Unconjugated (SL1867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Mouse spleen cells(blue). Primary Antibody (green line): Rabbit Anti-PD-1/PE-CY7 Conjugated antibody (SL1867R-PE-CY7) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 . Protocol The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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