Product Name	MANEA
Chinese Name	甘露糖苷酶2抗体
Alias	2-mannosidase; 4932703L02; Alpha 1,2 endomannosidase; DKFZp686D20120; Endo alpha D mannosidase; ENDO; Endo-alpha mannosidase; Endomannosidase; FLJ12838; Glycoprotein endo alpha 1,2 mannosidase; Glycoprotein endo-alpha-1; Glycoprotein endo-alpha-1,2-mannosidase; hEndo; Mandaselin; MANEA; MANEA_HUMAN; Mannosidase endo alpha; OTTMUSP0000000508; RP23-424K9.1.
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, Cat, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	54kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MANEA: 61-160/462
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008] Subcellular Location: Golgi apparatus membrane. Tissue Specificity: Highly expressed in the liver and kidney. Expressed at lower levels in muscle, pancreas, heart, placenta, lung and brain. Post-translational modifications: Undergoes proteolytic cleavage in the C-terminal region. Similarity: Belongs to the glycosyl hydrolase 99 family. SWISS: Q5SRI9 Gene ID: 79694 Database links: Entrez Gene: 79694 Human Entrez Gene: 242362 Mouse Entrez Gene: 140808 Rat Omim: 612327 Human SwissProt: Q5SRI9 Human SwissProt: Q6NXH2 Mouse SwissProt: Q5GF25 Rat Unigene: 533323 Human Unigene: 245602 Mouse Unigene: 10855 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MANEA) Polyclonal Antibody, Unconjugated (SL18652R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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