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Product Name MAN2C1 Chinese Name 甘露糖苷酶α胞浆抗体 Alias alpha D mannoside mannohydrolase; alpha mannosidase 2C1; Alpha mannosidase 6A8B; Alpha-D-mannoside mannohydrolase; Alpha-mannosidase 2C1; MA2C1_HUMAN; Man2c1; MAN6A8; MANA; MANA1; Mannosidase alpha 6A8; Mannosidase alpha A cytoplasmic; Mannosidase alpha class 2C member 1. Research Area Cell biology Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Chicken, Pig, Cow, Horse, Sheep, ) Applications IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 116kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MAN2C1: 61-160/1040 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Misfolded glycoproteins are deglycosylated by the peptide N-glycanase during the degradation process. Free oligosaccharides released by N-glycanase are catabolized by cytosolic MAN2C1, also designated alpha-mannosidase 2C1. MAN2C1, a member of the glycosyl hydrolase 38 family, can cleave alpha 1,2-linked, alpha 1,3-linked and alpha 1,6-linked mannose residues and is stimulated by cobalt. The furanose analogs, swainsonine (SW) and 1,4-dideoxy-1,4-imino-d-mannitol (DIM), are known inhibitors of MAN2C1. The inhibition of MAN2C1 can enhance the adhesion of Jurkat T cells, showing a cytoskeletal rearrangement of the cells.
Similarity:
Belongs to the glycosyl hydrolase 38 family.
SWISS:
Q9NTJ4
Gene ID:
4123
Database links:Entrez Gene: 4123 Human
Omim: 154580 Human
SwissProt: Q9NTJ4 Human
Unigene: 26232 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAN2C1) Polyclonal Antibody, Unconjugated (SL18649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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