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Product Name Vesicular Acetylcholine Transporter Chinese Name 囊泡乙酰胆碱通道抗体 Alias Vesicular Acetylcholine Transporter; MGC12716; rVAT; SLC18A3; Solute carrier family 18 (vesicular acetylcholine) member 3; Solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; VACht; VAChT; VACHT_HUMAN. literatures Specific References (1) | SL1833R has been referenced in 1 publications.[IF=2.894] Shen, Zhou. et al. Bone marrow mesenchymal stem cells therapy on bilateral pelvic nerve crush-induced voiding dysfunction in rats. INT UROGYNECOL J. 2022 Apr;:1-8 IF ; Rat.Research Area Tumour Cardiovascular Cell biology immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 57kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human VAT: 201-300/532 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Vesicular Acetylcholine Transporter (VAChT), (~70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate.
VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer’s disease and other nervous system disorders.
Function:
Involved in acetylcholine transport into synaptic vesicles.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Tissue Specificity:
Peripheral and central cholinergic nervous systems.
Similarity:
Belongs to the major facilitator superfamily.
Vesicular transporter family.
SWISS:
Q99536
Gene ID:
6572
Database links:Entrez Gene: 6572 Human
Entrez Gene: 20508 Mouse
Omim: 600336 Human
SwissProt: Q16572 Human
SwissProt: O35304 Mouse
Unigene: 654374 Human
Unigene: 190503 Mouse
Unigene: 9987 Rat
Product Picture Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Human SH-SY5Y cell lysates
Primary: Anti-Vesicular Acetylcholine Transporter (SL1833R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa
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