Product Name	PPARGC1A
Chinese Name	过氧化物酶体增殖物激活受体γ辅激活子1α抗体
Alias	LEM6; PGC-1 Alpha; PGC1 Alpha; Ligand effect modulator 6; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1 ; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; PGC 1 (alpha); PGC 1 alpha; PGC 1v; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPARGC 1 alpha.
literatures	Specific References  (33)     |     SL1832R has been referenced in 33 publications. [IF=14.528]   IF ;  mouse.   PubMed:32761924 [IF=14.528] Dejun Xu. et al. Melatonin protects mouse testes from palmitic acid‐induced lipotoxicity by attenuating oxidative stress and DNA damage in a SIRT1‐dependent manner. J Pineal Res. 2020 Nov;69(4):e12690  WB ;  Mouse.   PubMed:32761924 [IF=13.03] Chen, Yue, et al. "Nanosilver Incurs an Adaptive Shunt of Energy Metabolism Mode to Glycolysis in Tumor and Non-Tumor Cells." ACS Nano (2014).  WB ;  Human.   PubMed:24810997 [IF=7.666] Jia Wang. et al. A Role for PGC-1a in the Control of Abnormal Mitochondrial Dynamics in Alzheimer’s Disease. CELLS-BASEL. 2022 Jan;11(18):2849  WB, IF ;  Mouse.   PubMed:36139423 [IF=6.792] Yang Q et al. Hexavalent chromium induces mitochondrial dynamics disorder in rat liver by inhibiting AMPK/PGC-1α signaling pathway. Environ Pollut.2020 Oct;265(Pt A):114855.  WB ;  Rat.   PubMed:32474337 [IF=6.706] Yu Wang. et al. Seabuckthorn Reverses High-Fat-Diet-Induced Obesity and Enhances Fat Browning via Activation of AMPK/SIRT1 Pathway. NUTRIENTS. 2022 Jan;14(14):2903  WB ;  Mouse.   PubMed:35889860 [IF=6.529] Nan Chen. et al. β2-adrenoreceptor agonist ameliorates mechanical allodynia in paclitaxel-induced neuropathic pain via induction of mitochondrial biogenesis. Biomed Pharmacother. 2021 Dec;144:112331  IF ;  Rar.   PubMed:34673421 [IF=6.208] Min Liu. et al. Recruitment of Muscle Genes as an Effect of Brown Adipose Tissue Ablation in Cold-Acclimated Brandt’s Voles (Lasiopodomys brandtii). INT J MOL SCI. 2023 Jan;24(1):342  WB ;  Vole.   PubMed:36613791 [IF=6.055] Yun-Yi Zou. et al. Exercise intervention improves mitochondrial quality in non-alcoholic fatty liver disease zebrafish. FRONT ENDOCRINOL. 2023; 14: 1162485  WB ;  Zebrafish.   PubMed:37284220 [IF=6.048] He Zhang. et al. Feeding frequency affects glucose and lipid metabolism through SIRT1/AMPK pathway in growing pigs with the same amount of daily feed. J Nutr Biochem. 2021 Nov;:108919  WB ;  Pig.   PubMed:34843934 [IF=5.858] Yue Ding. et al. Protective role of sirtuin3 against oxidative stress and NLRP3 inflammasome in cholesterol accumulation and foam cell formation of macrophages with ox-LDL-stimulation. Biochem Pharmacol. 2021 Oct;192:114665  WB ;  Mouse.   PubMed:34181898 [IF=5.719] Yun-Yi Zou. et al. A High-Fat Diet Induces Muscle Mitochondrial Dysfunction and Impairs Swimming Capacity in Zebrafish: A New Model of Sarcopenic Obesity. NUTRIENTS. 2022 Jan;14(9):1975  WB ;  Zebrafish.   PubMed:35565942 [IF=5.455] Li, Xuling. et al. Intake of flavonoids from Astragalus membranaceus ameliorated brain impairment in diabetic mice via modulating brain-gut axis. Chin Med-Uk. 2022 Dec;17(1):1-19  WB,IF ;  Mouse.   PubMed:35151348 [IF=5.209] Xianglun Zhang. et al. Protective effect of resveratrol against hydrogen peroxide-induced oxidative stress in bovine skeletal muscle cells. Meat Sci. 2022 Mar;185:108724  WB ;  Cattle.   PubMed:34952489 [IF=5.202] Liu Lan Zhao. et al. Physiological responses to acute hypoxia in the liver of largemouth bass by alteration of mitochondrial function and Ca2+ exchange. AQUAT TOXICOL. 2023 Mar;256:106436  WB ;  Fish.   PubMed:36822139 [IF=5.168] Ruonan Wang. et al. The role of mitochondrial dynamics imbalance in hexavalent chromium-induced apoptosis and autophagy in rat testis. CHEM-BIOL INTERACT. 2023 Apr;374:110424  WB ;  Rat.   PubMed:36849043 [IF=4.02] Qin, Guohua, et al. "Sulfur dioxide inhalation stimulated mitochondrial biogenesis in rat brains." Toxicology 300.1 (2012): 67-74.  WB ;  Rat.   PubMed:22677886 [IF=3.998] Renli Qi. et al. The intestinal microbiota contributes to the growth and physiological state of muscle tissue in piglets. Sci Rep-Uk. 2021 May;11(1):1-14  WB ;  Pig.   PubMed:34045661 [IF=3.974] Ning X et al.Ambient PM2. 5 causes lung injuries and coupled energy metabolic disorder. (2018) Ecotoxicol. Environ. Saf. 170  WB ;  Mouse.   PubMed:30579162 [IF=3.647] Renli Qi. et al. MicroRNA-489-3p promotes adipogenesis by targeting the Postn gene in 3T3-L1 preadipocytes. Life Sci. 2021 Aug;278:119620  WB ;  Mouse.   PubMed:34004251 [IF=3.512] Piao Wang. et al. Effects of Root Extract of Morinda officinalis in Mice with High-Fat-Diet/Streptozotocin-Induced Diabetes and C2C12 Myoblast Differentiation. Acs Omega. 2021;6(41):26959–26968  WB ;  Mouse.   PubMed:34693116 [IF=3.448] Dai Y et al. Inhibition of miR‐23a‐3p promotes osteoblast proliferation and differentiation. J Cell Biochem. 2019 Nov 6.  IHC-P&WB ;  Rat.   PubMed:31691358 [IF=3.191] Jing Luo. et al. Cardiac Troponin I R193H Mutation Is Associated with Mitochondrial Damage in Cardiomyocytes. Dna Cell Biol. 2021 Feb;40(2):184-191  WB ;  Mouse.   PubMed:33465007 [IF=3.159] Wan Boyang. et al. Zearalenone promotes follicle development through activating SIRT1/PGC-1α signaling pathway in the ovaries of weaned gilts. J Anim Sci. 2022 Feb;:  WB,IHC ;  Pig(Gilt).   PubMed:10.1093/jas/skac058 [IF=3.046] Jia Wang. et al. Ameliorative effect of SIRT1 in postpartum depression mediated by upregulation of the glucocorticoid receptor. Neurosci Lett. 2021 Sep;761:136112  WB ;  Rat.   PubMed:34265417 [IF=2.678] Jingsong Xiao. et al. PGC-1ɑ Mediated-EXOG, a Specific Repair Enzyme for Mitochondrial DNA, Plays an Essential Role in the Rotenone-Induced Neurotoxicity of PC12 Cells. 2021 Jan 30  WB ;  Rat.   PubMed:33515431 [IF=2.639] Biqi Han. et al. Harmful Effects of Inorganic Mercury Exposure on Kidney Cells: Mitochondrial Dynamics Disorder and Excessive Oxidative Stress. 2021 May 31  WB ;  Human.   PubMed:34060062 [IF=2.495] Jinzhen Lv. et al. Thermo-TRP channels are involved in BAT thermoregulation in cold-acclimated Brandt's voles. COMP BIOCHEM PHYS B. 2022 Aug;:110794  WB ;  Vole.   PubMed:35964792 [IF=2.09] Lu, Jun-Juan, et al. "Tumor necrosis factor-like weak inducer of apoptosis regulates quadriceps muscle atrophy and fiber-type alteration in a rat model of chronic obstructive pulmonary disease." Tobacco Induced Diseases 15.1 (2017): 43.  WB ;  Rat.   PubMed:29151827 [IF=2.078] Shi L et al. β1 adrenoceptor antibodies induce myocardial apoptosis via inhibiting PGC-1α-related pathway. BMC Cardiovasc Disord. 2020 Jun 5;20(1):269.  WB ;  Rat.   PubMed:32503464
Research Area	immunology  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	88kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PGC-1: 601-700/798
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene. Function: Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Subunit: Binds MYBBP1A, which inhibits transcriptional activation by this protein. Interacts with PRDM16. Interacts with LRPPRC. Homooligomer. Interacts with LPIN1. Subcellular Location: Nucleus. Tissue Specificity: Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects. Post-translational modifications: hosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2. Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: Q9UBK2 Gene ID: 10891 Database links: Entrez Gene: 422815 Chicken Entrez Gene: 338446 Cow Entrez Gene: 479127 Dog Entrez Gene: 10891 Human Entrez Gene: 19017 Mouse Entrez Gene: 497232 Pig Entrez Gene: 83516 Rat Omim: 604517 Human SwissProt: Q865B7 Cow SwissProt: Q9UBK2 Human SwissProt: O70343 Mouse SwissProt: Q865B6 Pig SwissProt: Q9QYK2 Rat Unigene: 527078 Human Unigene: 259072 Mouse Unigene: 19172 Rat PGC-1α是一个新发现的转录激活因子,起到高游离脂肪酸刺激胰岛α细胞分泌的重要调控作用。
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-PPARGC1A (SL1832R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 90 kD Sample: Heart (Mouse) Lysate at 30 ug Primary: Anti- PPARGC1A (SL1832R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 88 kD Observed band size: 88 kD Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-PPARGC1A (SL1832R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 88 kD Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PPARGC1A/PGC1 alpha Polyclonal Antibody, Unconjugated(SL1832R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PPARGC1A antibody (SL1832R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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