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Product Name IL-16 Chinese Name 白介素16抗体 Alias Interleukin-16; HGNC:5980; HsT19289; Interleukin 16 precursor; LCF; Lymphocyte Chemotactic Factor; IL16; Lymphocyte chemoattractant factor; PrIL16; IL16_HUMAN. literatures Specific References (1) | SL1830R has been referenced in 1 publications.[IF=3.813] Hongwei Duan. et al. Dihydrotestosterone regulates oestrogen secretion, oestrogen receptor expression, and apoptosis in granulosa cells during antral follicle development. J Steroid Biochem. 2021 Mar;207:105819 WB,IHC ; Sheep.Research Area immunology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 13/76/147kDa Cellular localization The nucleus cytoplasmic Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human IL-16: 25-125/1332 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Function:
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
Subunit:
Homotetramer (Probable). Isoform 2 interacts with GRIN2A. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C.Isoform 2 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GABPB1. Isoform 2 interacts (via PDZ 3 domain) with HDAC3 (By similarity).
Subcellular Location:
Secreted (By similarity). Isoform 1: Cytoplasm. Note=Colocalizes with GRIN2C in neuronal cell bodies and neurites. Isoform 2: Cytoplasm. Nucleus (By similarity).
Tissue Specificity:
Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.
Post-translational modifications:
Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
Similarity:
Contains 4 PDZ (DHR) domains.
SWISS:
Q14005
Gene ID:
3603
Database links:Entrez Gene: 3603 Human
Entrez Gene: 16170 Mouse
Omim: 603035 Human
SwissProt: Q14005 Human
SwissProt: O54824 Mouse
Unigene: 459095 Human
Unigene: 10137 Mouse
Unigene: 104665 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-16) Polyclonal Antibody, Unconjugated (SL1830R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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