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Product Name phospho- LIM kinase 2 (Ser293) Chinese Name 磷酸化单丝氨酸蛋白激酶2抗体 Alias LIM kinase 2 (phospho S293); p-LIM kinase 2 (phospho S293); LIM domain kinase 2; LIMK 2; LIMK-2; LIMK2; LIMK2_HUMAN. Product Type Phosphorylated anti Research Area Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 72kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human LIM Kinase 1 around the phosphorylation site of Ser293): SK(p-S)PG Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Subcellular Location:
Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
Tissue Specificity:
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
Post-translational modifications:
Phosphorylated on serine and/or threonine residues by ROCK1.
Similarity:
Belongs to the protein kinase superfamily.
TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
SWISS:
P53671
Gene ID:
3985
Database links:Entrez Gene: 3985 Human
Entrez Gene: 16886 Mouse
Omim: 601988 Human
SwissProt: P53671 Human
SwissProt: O54785 Mouse
Unigene: 474596 Human
Unigene: 124176 Mouse
Unigene: 390323 Mouse
Unigene: 442189 Mouse
Unigene: 11013 Rat
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