Product Name	Leucyl tRNA synthetase
Chinese Name	胞浆亮氨酸tRNA连接酶抗体
Alias	Cytoplasmic leucyl tRNA synthetase; Cytosolic leucyl tRNA synthetase; FLJ10595; FLJ21788; hr025Cl; HSPC 192; HSPC192; KIAA1352; LARS 1; LARS; LARS1; Leucine translase; Leucine tRNA ligase; Leucine tRNA ligase; Leucyl tRNA synthetase cytoplasmic; LeuRS; LEUS; LRS; PIG 44; PIG44; Proliferation inducing gene 44; RNTLS.
Research Area	Cell biology  immunology  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	135kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Leucyl tRNA synthetase: 201-300/1176
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known. [provided by RefSeq, Jul 2008] Function: Leucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of leucine with leucyl tRNA. Subcellular Location: Cytoplasmic Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. SWISS: Q9P2J5 Gene ID: 51520 Database links: Entrez Gene: 51520 Human Entrez Gene: 107045 Mouse Omim: 151350 Human SwissProt: Q9P2J5 Human SwissProt: Q8BMJ2 Mouse Unigene: 432674 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Human colorectal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leucyl tRNA synthetase) Polyclonal Antibody, Unconjugated (SL18227R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leucyl tRNA synthetase) Polyclonal Antibody, Unconjugated (SL18227R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

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