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Product Name hUPF2/RENT2 Chinese Name hUPF2蛋白抗体 Alias DKFZp434D222; hUpf2; KIAA1408; MGC138834; MGC138835; Nonsense mRNA reducing factor 2; Regulator of nonsense transcripts 2; RENT2; RENT2_HUMAN; Smg 3; Smg 3 homolog nonsense mediated mRNA decay factor; Up-frameshift suppressor 2 homolog; UPF2; UPF2 regulator of nonsense transcripts homolog; UROC1; Urocanate hydratase; Yeast Upf2p homolog. Research Area Cell biology transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Cow, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 148kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human hUPF2/RENT2: 951-1050/1272 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq, Jul 2008]
Function:
Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA.
Subcellular Location:
Cytoplasm > perinuclear region.
Tissue Specificity:
Ubiquitous.
Similarity:
Contains 3 MIF4G domains.
SWISS:
Q9HAU5
Gene ID:
26019
Database links:Entrez Gene: 450304 Chimpanzee
Entrez Gene: 100069342 Horse
Entrez Gene: 26019 Human
Entrez Gene: 326622 Mouse
Omim: 605529 Human
SwissProt: Q9HAU5 Human
Unigene: 370689 Human
Unigene: 610110 Human
Unigene: 31102 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hUPF2) Polyclonal Antibody, Unconjugated (SL18106R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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