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Product Name SPIN4 Chinese Name 纺锤体样蛋白4抗体 Alias FLJ44984; RP11-93B10.1; SPIN4; SPIN4_HUMAN; Spindlin family, member 4; Spindlin-4. Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 29kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SPIN4: 1-100/249 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail SPIN4 (Spindlin Family Member 4) is a Protein Coding gene. An important paralog of this gene is SPIN1.
Similarity:
Belongs to the SPIN/STSY family.
SWISS:
Q56A73
Gene ID:
139886
Database links:Entrez Gene: 139886 Human
SwissProt: Q56A73 Human
Unigene: 612782 Human
Product Picture Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-SPIN4 (SL17668R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPIN4) Polyclonal Antibody, Unconjugated (SL17668R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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