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Product Name SPIN2B Chinese Name 纺锤体样蛋白2B抗体 Alias SPI2B_HUMAN; SPIN-2; SPIN-2B; SPIN2B; Spindlin-2B; Spindlin-like protein 2B. Research Area Cell biology Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SPIN2B: 1-50/258 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Involved in the regulation of cell cycle progression, this activity is related to the inhibition of apoptosis following the removal of essential growth factors.
Subcellular Location:
Nucleus.
Tissue Specificity:
Detected in all the examined tissues with highest expression in liver, followed by heart, stomach, kidney, skeletal muscle, placenta, and pancreas.
Similarity:
Belongs to the SPIN/STSY family.
SWISS:
Q9BPZ2
Gene ID:
474343
Database links:Entrez Gene: 474343 Human
Omim: 300517 Human
SwissProt: Q9BPZ2 Human
SwissProt: Q5RA80 Orangutan
Unigene: 460717 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPIN2B) Polyclonal Antibody, Unconjugated (SL17666R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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