Product Name	phospho-SPHK2 (Thr614)
Chinese Name	磷酸化鞘氨醇激酶2抗体
Alias	SPHK2 (phospho T614); p-SPHK2 (phospho T614); SK 2; Sphingosine kinase 2; Sphingosine kinase type 2; SPHK2; SPHK2_HUMAN; SPK 2.
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction  Kinases and Phosphatases  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	72kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human SPHK2 around the phosphorylation site of Thr614: PL(p-T)PR
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. Subcellular Location: Cytoplasm. Membrane and Lysosome membrane. Similarity: Contains 1 DAGKc domain. SWISS: Q9NRA0 Gene ID: 56848 Database links: Entrez Gene: 56848 Human Entrez Gene: 56632 Mouse Entrez Gene: 308589 Rat Omim: 607092 Human SwissProt: Q9NRA0 Human SwissProt: Q9JIA7 Mouse Unigene: 528006 Human Unigene: 24222 Mouse Unigene: 41053 Rat
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Siha(huamn) Cell Lysate at 40 ug Primary: Anti-p-SPHK2(Thr614) (SL17661R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 75 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-SPHK2 (Thr614)) Polyclonal Antibody, Unconjugated (SL17661R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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