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Product Name SPACA3 Chinese Name 精子顶体膜相关蛋白3抗体 Alias 1700025M08Rik; ALLP17; Cancer/testis antigen 54; sperm acrosome associated 3; CT54; LYC3; Lysozyme like sperm specific secretory protein ALLP17; Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17; Lysozyme-like protein 3; LYZL3; processed form; SACA3_HUMAN; SLLP1; SPACA3; Sperm acrosome associated 3; Sperm acrosome membrane-associated protein 3; Sperm lysozyme-like protein 1; Sperm protein reactive with antisperm antibodies; Sperm protein reactive with ASA; 1. Research Area Tumour Cell biology Developmental biology Signal transduction The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 23kDa Cellular localization The cell membrane Extracellular matrix Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SPACA3: 51-150/215 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail SPACA3 is a 215 amino acid protein that participates in the fusion and adhesion of sperm and egg plasma membrane during fertilization. Identified as a novel cancer/testis antigen in hematologic malignancies, SPACA3 has the ability to elicit B-cell immune responses in patients with cancer and is considered a potential target for immunotherapy. A member of the glycosyl hydrolase 22 family which is expressed in testis, placenta and epididymis, SPACA3 exists as two alternatively spliced isoforms; SPACA3 isoform 1 is a single-pass type II membrane protein of the sperm acrosome whereas SPACA3 isoform 2 is a secreted protein.
Function:
Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.
Subcellular Location:
Secreted and Cytoplasmic vesicle > secretory vesicle > acrosome membrane. Anterior acrosome in non-capacitated spermatozoa and retained in the equatorial segment and in the luminal face of both the inner and outer acrosomal membranes following capacitation and the acrosome reaction.
Tissue Specificity:
The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.
Post-translational modifications:
The processed form derives from the membrane form by proteolytic processing.
Similarity:
Belongs to the glycosyl hydrolase 22 family.
SWISS:
Q8IXA5
Gene ID:
124912
Database links:Entrez Gene: 124912 Human
Omim: 612749 Human
SwissProt: Q8IXA5 Human
Unigene: 434112 Human
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