Product Name	phospho-JNK1 (Thr183)
Chinese Name	磷酸化氨基末端激酶1抗体
Alias	JNK1 (phospho T183); p-JNK1 (phospho T183); AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK 1; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; mapk8; Mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; MK08_HUMAN; p54 gamma; Prkm8; Protein kinase JNK1; Protein kinase, mitogen-activated, 8; SAPK 1; SAPK gamma; SAPK1; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1.
literatures	Specific References  (16)     |     SL17591R has been referenced in 16 publications. [IF=9.473] Shuting Wei. et al. Particle matters induce airway epithelial barrier dysfunction in vivo and in vitro: from a more realistic inhalation scenario. ENVIRON SCI-NANO. 2022 Jun;:  WB ;  Human.   PubMed:10.1039/D2EN00390B [IF=7.963] Meiqiong Wu. et al. Suppression of NADPH oxidase 4 inhibits PM2.5-induced cardiac fibrosis through ROS-P38 MAPK pathway. SCI TOTAL ENVIRON. 2022 Apr;:155558  WB ;  Mouse,Rat.   PubMed:35504386 [IF=5.293] Liu Jiawei. et al. Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-κB and MAPKs Signaling in Bovine Endometrial Epithelial Cells. Front Cell Infect Mi. 2022 Mar;0:199  WB ;  Cattle.   PubMed:35310848 [IF=4.996] Cong Changsheng. et al. Renin-angiotensin system inhibitors mitigate radiation pneumonitis by activating ACE2-angiotensin-(1–7) axis via NF-κB/MAPK pathway. SCI REP-UK. 2023 May;13(1):1-11  WB ;  Mouse.   PubMed:37221286 [IF=4.478] Dan-Ping Xie. et al. Peroxiredoxin 5 protects HepG2 cells from ethyl β-carboline-3-carboxylate-induced cell death via ROS-dependent MAPK signalling pathways. J CANCER. 2022 Sep 6;13(11):3258-3267  WB ;  Human.   PubMed:36118528 [IF=4.064] Ming Ni. et al. Sox11 Modified Tendon-Derived Stem Cells Promote the Repair of Osteonecrosis of Femoral Head:. Cell Transplant. 2021;():  WB ;  Rabbit.   PubMed:34699265 [IF=4.014] M. Zhang. et al. Osteocalcin reduces fat accumulation and inflammatory reaction by inhibiting ROS-JNK signal pathway in chicken embryonic hepatocytes. POULTRY SCIENCE. 2022 Jun;:102026  WB ;   PubMed:10.1016/j.psj.2022.102026 [IF=3.829] Gao, Pei. et al. S1PR1 regulates NDV-induced IL-1β expression via NLRP3/caspase-1 inflammasome. VET RES. 2022 Dec;53(1):1-13  WB ;  Chicken.   PubMed:35854395 [IF=3.352] Yusong Miao. et al. Methylsulfonylmethane ameliorates inflammation via NF-κB and ERK/JNK-MAPK signaling pathway in chicken trachea and HD11 cells during Mycoplasma gallisepticum infection. Poultry Sci. 2022 Jan;:101706  WB ;  Chicken.   PubMed:35121233 [IF=3.166] Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124.  WB ;  Piglet.   PubMed:30055240 [IF=2.741] Zhang, Di. et al. Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-κB and MAPK signaling pathways. BMC VET RES. 2022 Dec;18(1):1-11  WB ;  Rat.   PubMed:35624447 [IF=2.094] Jing Li. et al. Melatonin ameliorates cypermethrin-induced impairments by regulating oxidative stress, DNA damage and apoptosis in porcine Sertoli cells. Theriogenology. 2021 Mar;:  WB ;  Pig.   PubMed:10.1016/j.theriogenology.2021.03.011 [IF=2.027] Li QH et al. Effect of heat stress on mitogen-activated protein kinases in the hypothalamic− pituitary− gonadal axis of developing Wenchang chicks. Poultry Science.2019.  IHC-P&WB ;  chick.   PubMed:doi:10.3382/ps/pez499 [IF=1.723] Wang X et al. Wilms' tumour 1 (WT1) negatively regulates the expression of connexin 43 via a non-canonical Wnt signalling pathway in cultured bovine Sertoli cells. Reprod Fertil Dev. 2020 Feb 6.  WB ;  Bovine.   PubMed:32023428 [IF=1.713] Jian Wang. et al. Lactobacillus salivarius ameliorated Mycoplasma gallisepticum-induced inflammatory injury and secondary Escherichia coli infection in chickens: Involvement of intestinal microbiota. Vet Immunol Immunop. 2021 Mar;233:110192  WB ;  Chicken.   PubMed:33476924 [IF=1.713] Jian Wang. et al. A respiratory commensal bacterium acts as a risk factor for Mycoplasma gallisepticum infection in chickens. Vet Immunol Immunop. 2020 Dec;230:110127  WB ;  Chicken.   PubMed:33080531
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Zebrafish, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183: MM(p-T)PY
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013] Function: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. Post-translational modifications: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. SWISS: P45983 Gene ID: 5599 Database links: Entrez Gene: 5599 Human Entrez Gene: 26419 Mouse Entrez Gene: 116554 Rat Omim: 601158 Human SwissProt: P45983 Human SwissProt: Q91Y86 Mouse SwissProt: P49185 Rat Unigene: 138211 Human Unigene: 21495 Mouse Unigene: 4090 Rat
Product Picture	Sample: Hela-UV(Human) Cell Lysate at 30 ug 293T-UV(Human) Cell Lysate at 30 ug Jurkat-UV(Human) Cell Lysate at 30 ug Primary: Anti-phospho-JNK1 (Thr183) (SL17591R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-phospho-JNK1 (Thr183) (SL17591R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-JNK1 (Thr183)) Polyclonal Antibody, Unconjugated (SL17591R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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