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Product Name EAAT3 Chinese Name 胶质细胞谷氨酸运载蛋白3/神经/epithelial cells谷氨酸运载蛋白抗体 Alias Excitatory amino acid transporters 3; Slc1a1; Eaac1; Eaat3; SLC1A1; EAAC1; EAAT3; solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1; EAAC 2; Excitatory amino acid carrier 1; Excitatory amino acid carrier 2; Excitatory amino acid carrier1; MEAAC 1; MEAAC1; Neuronal and epithelial glutamate transporter; REAAC 1; REAAC1; Slc1 a1; Slc1a 1; Slc1a1; Sodium dependent glutamate/aspartate transporter 3; Solute carrier family 1, member 1; EAA3_HUMAN. literatures Specific References (1) | SL1752R has been referenced in 1 publications.[IF=4.053] Ham J et al. Ginsenoside Rg3 and Korean red ginseng extract epigenetically regulate the tumor-related long noncoding RNAs RFX3-AS1 and STXBP5-AS1.Journal of Ginseng Research. doi:10.1016/j.jgr.2019.02.004 WB ; Human.Research Area immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 58kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human EAAT3: 451-524/524 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.
Function:
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5 (By similarity).
Subunit:
Interacts with ARL6IP5/PRAF3.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Tissue Specificity:
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
Post-translational modifications:
Glycosylated.
Similarity:
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.
SWISS:
P43005
Gene ID:
6505
Database links:Entrez Gene: 6505 Human
Entrez Gene: 20510 Mouse
Omim: 133550 Human
SwissProt: P43005 Human
SwissProt: P51906 Mouse
Unigene: 444915 Human
Unigene: 246670 Mouse
Unigene: 6384 Rat
胶质细胞谷氨酸运载蛋白3又称:神经/epithelial cells谷氨酸运载蛋白Product Picture Blank control: Mouse splen.
Primary Antibody (green line): Rabbit Anti-EAAT3 antibody (SL1752R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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