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Product Name ORP1 Chinese Name 氧固醇Binding protein样1A抗体 Alias FLJ10217; ORP 1; ORP-1; ORP1; OSBL1_HUMAN; OSBP 8; OSBP L1; OSBP related protein 1; OSBP-related protein 1; OSBP8; OSBPL 1; OSBPL 1A; OSBPL 1B; OSBPL1; OSBPL1A; OSBPL1B; Oxysterol binding protein like 1A; Oxysterol binding protein like 1B; Oxysterol binding protein related protein 1; Oxysterol-binding protein-related protein 1. literatures Specific References (1) | SL17514R has been referenced in 1 publications.[IF=4.175] Li Liang. et al. Identification of key genes involved in tumor immune cell infiltration and cetuximab resistance in colorectal cancer. Cancer Cell Int. 2021 Dec;21(1):1-22 IHC ;Research Area Tumour Cell biology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 108kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ORP1: 851-950/950 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq, Jul 2008]
Function:
Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate.
Subunit:
Interacts with VAPA (By similarity). Interacts with the GTP-bound form of RAB7A. Interacts with OAS1B (By similarity).
Subcellular Location:
Late endosome. Note=Colocalizes with RAB7A, RAB9A and LAMP1 in late endosomes.
Similarity:
Belongs to the OSBP family.
Contains 3 ANK repeats.
Contains 1 PH domain.
SWISS:
Q9BXW6
Gene ID:
114876
Database links:Entrez Gene: 114876 Human
Entrez Gene: 64291 Mouse
Omim: 606730 Human
SwissProt: Q9BXW6 Human
SwissProt: Q91XL9 Mouse
Unigene: 370725 Human
Unigene: 259470 Mouse
Unigene: 443224 Mouse
Unigene: 178664 Rat
Product Picture Protein: brain(mouse) lystes at 40ug;
Primary: Anti-ORP1 (SL17514R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size :108 kD
Observed band size :108 kD
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ORP1 Polyclonal Antibody, Unconjugated(SL17514R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HAVCR1 Polyclonal Antibody, Unconjugated(SL17514R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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