Product Name	MAPKAP1/SIN1
Chinese Name	丝裂原活化蛋白激酶相关蛋白1抗体
Alias	MAPKAP 1; MAPKAP1; MEKK2 interacting protein 1; MGC2745; MIP 1; MIP1; Mitogen activated protein kinase associated protein 1; Mitogen-activated protein kinase 2-associated protein 1; mSIN1; OTTHUMP00000064207; Ras inhibitor MGC2745; SAPK interacting protein 1; SAPK-interacting protein 1; SIN 1; SIN1_HUMAN; SIN1b; SIN1g; Stress activated map kinase interacting protein 1; Stress activated protein kinase interacting 1; Stress-activated map kinase-interacting protein 1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1.
Research Area	Cell biology  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	30/50/65 (59)kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MAPKAP1/SIN1: 61-160/522
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008] Function: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Subcellular Location: Cell membrane. Cytoplasmic vesicle. Nucleus. Tissue Specificity: Ubiquitously expressed, with highest levels in heart and skeletal muscle. Similarity: Belongs to the SIN1 family. SWISS: Q9BPZ7 Gene ID: 79109 Database links: Entrez Gene: 395627 Chicken Entrez Gene: 79109 Human Entrez Gene: 227743 Mouse Entrez Gene: 296648 Rat Omim: 610558 Human SwissProt: Q9W6S3 Chicken SwissProt: Q9BPZ7 Human SwissProt: Q8BKH7 Mouse SwissProt: Q6AYF1 Rat Unigene: 495138 Human Unigene: 270866 Mouse Unigene: 105886 Rat
Product Picture	Sample: Heart (Mouse)Lysate at 40 ug Primary: Anti-MAPKAP1/SIN1 (SL17496R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30/50/65 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAPKAP1/SIN1) Polyclonal Antibody, Unconjugated (SL17496R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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