Product Name	CYP3A4
Chinese Name	细胞色素P450 3A4抗体
Alias	Cytochrome p450 3A4; CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA4; CYPIIIA3; Cytochrome P450 family 3 subfamily A polypeptide 4; Cytochrome P450 subfamily IIIA polypeptide 4; Glucocorticoid inducible P450; HLP; MGC126680; NF 25; NF25; P450 III steroid inducible; P450 PCN1; P450C3; P450PCN1; Quinine 3 monooxygenase; Taurochenodeoxycholate 6 alpha hydroxylase; CP3A4_HUMAN; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; Albendazole sulfoxidase; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase; Taurochenodeoxycholate 6-alpha-hydroxylase.
literatures	Specific References  (4)     |     SL1742R has been referenced in 4 publications. [IF=6.208] Bin Yang. et al. HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice. INT J MOL SCI. 2023 Jan;24(6):5686  ICC ;  Rat.   PubMed:36982757 [IF=3.83] Jiang et al. Development of an IgY Antibody-Based Immunoassay for the Screening of the CYP2E1 Inhibitor/Enhancer from Herbal Medicines. (2017) Front.Pharmacol. 7:502  WB ;  Mouse.   PubMed:28066249 [IF=2.56] Ito, Junitsu, et al. ʺAscorbic acid reverses the prolonged anesthetic action of pentobarbital in Akr1a knockout mice.ʺ Life Sciences (2013).  WB ;  Mouse.   PubMed:24355294 [IF=2.302] Sun B et al. The interleukin-17 G-197A polymorphism is associated with cyclosporine metabolism and transplant rejection in liver transplant recipients. Pharmacogenomics. 2019 Feb 25.  WB ;  Human.   PubMed:30799725
Research Area	Tumour  Cell biology  The new supersedes the old  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	55kDa
Cellular localization	cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CYP3A4: 18-120/503
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011] Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide. Subcellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein. Tissue Specificity: Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). Post-translational modifications: Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro). Similarity: Belongs to the cytochrome P450 family. SWISS: P08684 Gene ID: 1576 Database links: Entrez Gene: 1576 Human Omim: 124010 Human SwissProt: P08684 Human Unigene: 728751 Human 细胞色素3A4(CYP3A4)是细胞色素P450 超家族成员之一, 主要存在于肝脏中和小肠中。它是人类药物代谢酶中的最重要的酶, CYP3A4与外源性致癌物、抗癌药物和内源性类固醇激素、维生素、脂肪酸的代谢转化密切相关。
Product Picture	Sample: Lane1: Brain(Rat) Lysate at 30 ug Lane2: Intestine(Mouse) Lysate at 30 ug Primary: Anti-CYP3A4 (SL1742R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 55kD Observed band size : 50kD Tissue/cell: Human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CYP3A4 Polyclonal Antibody, Unconjugated(SL1742R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CYP3A4) polyclonal Antibody, Unconjugated (SL1742R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: Raji. Primary Antibody (green line): Rabbit Anti-CYP3A4 antibody (SL1742R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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